{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ravi"],"funding":["Science and Engineering Research Board","Department of Biotechnology, Government of West Bengal","ODCDC CDC HHS","NIH HHS"],"pagination":["e114093"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10646554"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(22)"],"pubmed_abstract":["Owing to their capability to disrupt the oxidative protein folding environment in the endoplasmic reticulum (ER), thiol antioxidants, such as dithiothreitol (DTT), are used as ER-specific stressors. We recently showed that thiol antioxidants modulate the methionine-homocysteine cycle by upregulating an S-adenosylmethionine-dependent methyltransferase, rips-1, in Caenorhabditis elegans. However, the changes in cellular physiology induced by thiol stress that modulate the methionine-homocysteine cycle remain uncharacterized. Here, using forward genetic screens in C. elegans, we discover that thiol stress enhances rips-1 expression via the hypoxia response pathway. We demonstrate that thiol stress activates the hypoxia response pathway. The activation of the hypoxia response pathway by thiol "],"journal":["The EMBO journal"],"pubmed_title":["Thiol reductive stress activates the hypoxia response pathway."],"pmcid":["PMC10646554"],"funding_grant_id":["BT/RLF/Re‐entry/50/2020","P40 OD010440","SRG/2020/000022"],"pubmed_authors":["Singh J","Ravi","Bhattacharyya S","Kumar A"],"additional_accession":[]},"is_claimable":false,"name":"Thiol reductive stress activates the hypoxia response pathway.","description":"Owing to their capability to disrupt the oxidative protein folding environment in the endoplasmic reticulum (ER), thiol antioxidants, such as dithiothreitol (DTT), are used as ER-specific stressors. We recently showed that thiol antioxidants modulate the methionine-homocysteine cycle by upregulating an S-adenosylmethionine-dependent methyltransferase, rips-1, in Caenorhabditis elegans. However, the changes in cellular physiology induced by thiol stress that modulate the methionine-homocysteine cycle remain uncharacterized. Here, using forward genetic screens in C. elegans, we discover that thiol stress enhances rips-1 expression via the hypoxia response pathway. We demonstrate that thiol stress activates the hypoxia response pathway. The activation of the hypoxia response pathway by thiol ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-06-06T22:14:45.06Z","creation":"2025-04-04T00:39:08.061Z"},"accession":"S-EPMC10646554","cross_references":{"pubmed":["37902464"],"doi":["10.15252/embj.2023114093"]}}