biostudies-literatureBikuna-Izagirre MGobierno espa?ol, Ministerio de Econom?a e IndustriaInstituto de Salud Carlos IIIEuropean CommissionEuropean Regional Development Fund6333-6344https://www.ebi.ac.uk/biostudies/studies/S-EPMC10646841biostudies-literatureUnknown9(11)Primary open-angle glaucoma is characterized by the progressive degeneration of the optic nerve, with the high intraocular pressure (IOP) being one of the main risk factors. The human trabecular meshwork (HTM), specifically the juxtacanalicular tissue (JCT), is responsible for placing resistance to the aqueous humor (AH) outflow and the resulting IOP control. Currently, the lack of a proper <i>in vitro</i> JCT model and the complexity of three-dimensional models impede advances in understanding the relationship between AH outflow and HTM degeneration. Therefore, we design an <i>in vitro</i> JCT model using a polycaprolactone (PCL) nanofibrous scaffold, which supports cells to recapitulate the functional JCT morphology and allow the study of outflow physiology. Mechanical and morphological ACS biomaterials science & engineeringNanofibrous PCL-Based Human Trabecular Meshwork for Aqueous Humor Outflow Studies.PMC10646841UE17259601Extramiana LAldazabal JBikuna-Izagirre MCarnero EMoreno-Montanes JParedes JfalseNanofibrous PCL-Based Human Trabecular Meshwork for Aqueous Humor Outflow Studies.Primary open-angle glaucoma is characterized by the progressive degeneration of the optic nerve, with the high intraocular pressure (IOP) being one of the main risk factors. The human trabecular meshwork (HTM), specifically the juxtacanalicular tissue (JCT), is responsible for placing resistance to the aqueous humor (AH) outflow and the resulting IOP control. Currently, the lack of a proper <i>in vitro</i> JCT model and the complexity of three-dimensional models impede advances in understanding the relationship between AH outflow and HTM degeneration. Therefore, we design an <i>in vitro</i> JCT model using a polycaprolactone (PCL) nanofibrous scaffold, which supports cells to recapitulate the functional JCT morphology and allow the study of outflow physiology. Mechanical and morphological 2023-01-01T00:00:00Z2023 Nov2025-04-26T17:15:21.991Z2025-04-06T15:30:13.29ZS-EPMC106468413772556110.1021/acsbiomaterials.3c01071