biostudies-literatureTriaille CA.R.CFondation Saint-LucCAP48 RTBFWalloon excellence in life sciences and biotechnologyFonds De La Recherche Scientifique - FNRS1538-1546https://www.ebi.ac.uk/biostudies/studies/S-EPMC10646909biostudies-literatureUnknown82(12)<h4>Objectives</h4>Transcriptomic profiling of synovial tissue from patients with early, untreated rheumatoid arthritis (RA) was used to explore the ability of unbiased, data-driven approaches to define clinically relevant subgroups.<h4>Methods</h4>RNASeq was performed on 74 samples, with disease activity data collected at inclusion. Principal components analysis (PCA) and unsupervised clustering were used to define patient clusters based on expression of the most variable genes, followed by pathway analysis and inference of relative abundance of immune cell subsets. Histological assessment and multiplex immunofluorescence (for CD45, CD68, CD206) were performed on paraffin sections.<h4>Results</h4>PCA on expression of the (n=894) most variable genes across this series did not divide sampleAnnals of the rheumatic diseasesDisease activity drives transcriptomic heterogeneity in early untreated rheumatoid synovitis.PMC1064690919/24–098N/ACDR J.0124.22WELBIO-CR-2019A-03RTilman GDurez PSokolova TBouzin CDe Montjoye SMeric de Bellefon LTriaille CLoriot AMarchandise JLauwerys BRNzeusseu-Toukap ALimaye NGalant CfalseDisease activity drives transcriptomic heterogeneity in early untreated rheumatoid synovitis.<h4>Objectives</h4>Transcriptomic profiling of synovial tissue from patients with early, untreated rheumatoid arthritis (RA) was used to explore the ability of unbiased, data-driven approaches to define clinically relevant subgroups.<h4>Methods</h4>RNASeq was performed on 74 samples, with disease activity data collected at inclusion. Principal components analysis (PCA) and unsupervised clustering were used to define patient clusters based on expression of the most variable genes, followed by pathway analysis and inference of relative abundance of immune cell subsets. Histological assessment and multiplex immunofluorescence (for CD45, CD68, CD206) were performed on paraffin sections.<h4>Results</h4>PCA on expression of the (n=894) most variable genes across this series did not divide sample2023-01-01T00:00:00Z2023 Dec2026-06-18T06:45:38.2Z2025-04-04T11:01:12.36ZS-EPMC106469093750720110.1136/ard-2023-224068