{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(46)"],"submitter":["Rajan AM"],"pubmed_abstract":["Despite their importance in tissue maintenance and repair, fibroblast diversity and plasticity remain poorly understood. Using single-cell RNA sequencing, we uncover distinct sclerotome-derived fibroblast populations in zebrafish, including progenitor-like perivascular/interstitial fibroblasts, and specialized fibroblasts such as tenocytes. To determine fibroblast plasticity in vivo, we develop a laser-induced tendon ablation and regeneration model. Lineage tracing reveals that laser-ablated tenocytes are quickly regenerated by preexisting fibroblasts. By combining single-cell clonal analysis and live imaging, we demonstrate that perivascular/interstitial fibroblasts actively migrate to the injury site, where they proliferate and give rise to new tenocytes. By contrast, perivascular fibrob"],"journal":["Science advances"],"pagination":["eadi5771"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10651129"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell analysis reveals distinct fibroblast plasticity during tenocyte regeneration in zebrafish."],"pmcid":["PMC10651129"],"pubmed_authors":["Rajan AM","Rosin NL","Biernaskie J","Labit E","Liao S","Huang P"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell analysis reveals distinct fibroblast plasticity during tenocyte regeneration in zebrafish.","description":"Despite their importance in tissue maintenance and repair, fibroblast diversity and plasticity remain poorly understood. Using single-cell RNA sequencing, we uncover distinct sclerotome-derived fibroblast populations in zebrafish, including progenitor-like perivascular/interstitial fibroblasts, and specialized fibroblasts such as tenocytes. To determine fibroblast plasticity in vivo, we develop a laser-induced tendon ablation and regeneration model. Lineage tracing reveals that laser-ablated tenocytes are quickly regenerated by preexisting fibroblasts. By combining single-cell clonal analysis and live imaging, we demonstrate that perivascular/interstitial fibroblasts actively migrate to the injury site, where they proliferate and give rise to new tenocytes. By contrast, perivascular fibrob","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-19T16:14:46.602Z","creation":"2025-04-19T16:14:46.602Z"},"accession":"S-EPMC10651129","cross_references":{"pubmed":["37967180"],"doi":["10.1126/sciadv.adi5771"]}}