{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang S"],"funding":["Guangdong Medical Research Foundation","National Natural Science Foundation of China","Guangdong Provincial Hospital of Traditional Chinese Medicine"],"pagination":["e21658"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10651509"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(11)"],"pubmed_abstract":["As an important virulence phenotype of <i>Escherichia coli</i>, the regulation mechanism of biofilm by non-coding RNA and quorum sensing system has not been clarified. Here, by transcriptome sequencing and RT-PCR analysis, we found CsrB, a non-coding RNA of the carbon storage regulation system, was positively regulated by the LuxR protein SdiA. Furthermore, β-galactosidase reporter assays showed that SdiA enhanced promoter transcriptional activity of <i>csrB</i>. The consistent dynamic expression levels of SdiA and CsrB during <i>Escherichia coli</i> growth were also detected. Moreover, curli assays and biofilm assays showed <i>sdiA</i> deficiency in <i>Escherichia coli SM10λπ</i> or <i>BW25113</i> led to a decreased formation of biofilm, and was significantly restored by over-expression o"],"journal":["Heliyon"],"pubmed_title":["Quorum sensing <i>N</i>-acyl homoserine lactones-SdiA enhances the biofilm formation of <i>E. coli</i> by regulating sRNA CsrB expression."],"pmcid":["PMC10651509"],"funding_grant_id":["A2020560","82072345","YN2018QJ01","YN2019MJ01","81672081","YN2019QJ03","81801973"],"pubmed_authors":["Liu J","Lu Y","Xiong R","Li Q","Zhang S","Chen C","Zhang W","Li Y","Li S","Long Y","Zhang Y","Shu Y","Xu Z"],"additional_accession":[]},"is_claimable":false,"name":"Quorum sensing <i>N</i>-acyl homoserine lactones-SdiA enhances the biofilm formation of <i>E. coli</i> by regulating sRNA CsrB expression.","description":"As an important virulence phenotype of <i>Escherichia coli</i>, the regulation mechanism of biofilm by non-coding RNA and quorum sensing system has not been clarified. Here, by transcriptome sequencing and RT-PCR analysis, we found CsrB, a non-coding RNA of the carbon storage regulation system, was positively regulated by the LuxR protein SdiA. Furthermore, β-galactosidase reporter assays showed that SdiA enhanced promoter transcriptional activity of <i>csrB</i>. The consistent dynamic expression levels of SdiA and CsrB during <i>Escherichia coli</i> growth were also detected. Moreover, curli assays and biofilm assays showed <i>sdiA</i> deficiency in <i>Escherichia coli SM10λπ</i> or <i>BW25113</i> led to a decreased formation of biofilm, and was significantly restored by over-expression o","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-04T09:30:09.141Z","creation":"2025-04-04T09:30:09.141Z"},"accession":"S-EPMC10651509","cross_references":{"pubmed":["38027585"],"doi":["10.1016/j.heliyon.2023.e21658"]}}