biostudies-literatureZhang SGuangdong Medical Research FoundationNational Natural Science Foundation of ChinaGuangdong Provincial Hospital of Traditional Chinese Medicinee21658https://www.ebi.ac.uk/biostudies/studies/S-EPMC10651509biostudies-literatureUnknown9(11)As an important virulence phenotype of <i>Escherichia coli</i>, the regulation mechanism of biofilm by non-coding RNA and quorum sensing system has not been clarified. Here, by transcriptome sequencing and RT-PCR analysis, we found CsrB, a non-coding RNA of the carbon storage regulation system, was positively regulated by the LuxR protein SdiA. Furthermore, β-galactosidase reporter assays showed that SdiA enhanced promoter transcriptional activity of <i>csrB</i>. The consistent dynamic expression levels of SdiA and CsrB during <i>Escherichia coli</i> growth were also detected. Moreover, curli assays and biofilm assays showed <i>sdiA</i> deficiency in <i>Escherichia coli SM10λπ</i> or <i>BW25113</i> led to a decreased formation of biofilm, and was significantly restored by over-expression oHeliyonQuorum sensing <i>N</i>-acyl homoserine lactones-SdiA enhances the biofilm formation of <i>E. coli</i> by regulating sRNA CsrB expression.PMC10651509A202056082072345YN2018QJ01YN2019MJ0181672081YN2019QJ0381801973Liu JLu YXiong RLi QZhang SChen CZhang WLi YLi SLong YZhang YShu YXu ZfalseQuorum sensing <i>N</i>-acyl homoserine lactones-SdiA enhances the biofilm formation of <i>E. coli</i> by regulating sRNA CsrB expression.As an important virulence phenotype of <i>Escherichia coli</i>, the regulation mechanism of biofilm by non-coding RNA and quorum sensing system has not been clarified. Here, by transcriptome sequencing and RT-PCR analysis, we found CsrB, a non-coding RNA of the carbon storage regulation system, was positively regulated by the LuxR protein SdiA. Furthermore, β-galactosidase reporter assays showed that SdiA enhanced promoter transcriptional activity of <i>csrB</i>. The consistent dynamic expression levels of SdiA and CsrB during <i>Escherichia coli</i> growth were also detected. Moreover, curli assays and biofilm assays showed <i>sdiA</i> deficiency in <i>Escherichia coli SM10λπ</i> or <i>BW25113</i> led to a decreased formation of biofilm, and was significantly restored by over-expression o2023-01-01T00:00:00Z2023 Nov2025-04-04T09:30:09.141Z2025-04-04T09:30:09.141ZS-EPMC106515093802758510.1016/j.heliyon.2023.e21658