{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Klement K"],"funding":["Deutsche Forschungsgemeinschaft","Wilhelm Sander-Stiftung","Friedrich-Alexander-Universität Erlangen-Nürnberg","IZKF"],"pagination":["jcs261214"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10652037"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["136(20)"],"pubmed_abstract":["Axin (also known as AXIN1) is a central negative regulator of the proto-oncogenic Wnt/β-catenin signaling pathway, as axin condensates provide a scaffold for the assembly of a multiprotein complex degrading β-catenin. Axin, in turn, is degraded through tankyrase. Consequently, tankyrase small-molecule inhibitors block Wnt signaling by stabilizing axin, revealing potential for cancer therapy. Here, we discovered that axin is phosphorylated by casein kinase 1 alpha 1 (CSNK1A1, also known as CK1α) at an N-terminal casein kinase 1 consensus motif, and that this phosphorylation is antagonized by the catalytic subunit alpha of protein phosphatase 1 (PPP1CA, hereafter referred to as PP1). Axin condensates promoted phosphorylation by enriching CK1α over PP1. Importantly, the phosphorylation took p"],"journal":["Journal of cell science"],"pubmed_title":["Phosphorylation of axin within biomolecular condensates counteracts its tankyrase-mediated degradation."],"pmcid":["PMC10652037"],"funding_grant_id":["D30","BE 7055/2-1","2018.017.2"],"pubmed_authors":["Bruckner M","Klement K","Bernkopf DB"],"additional_accession":[]},"is_claimable":false,"name":"Phosphorylation of axin within biomolecular condensates counteracts its tankyrase-mediated degradation.","description":"Axin (also known as AXIN1) is a central negative regulator of the proto-oncogenic Wnt/β-catenin signaling pathway, as axin condensates provide a scaffold for the assembly of a multiprotein complex degrading β-catenin. Axin, in turn, is degraded through tankyrase. Consequently, tankyrase small-molecule inhibitors block Wnt signaling by stabilizing axin, revealing potential for cancer therapy. Here, we discovered that axin is phosphorylated by casein kinase 1 alpha 1 (CSNK1A1, also known as CK1α) at an N-terminal casein kinase 1 consensus motif, and that this phosphorylation is antagonized by the catalytic subunit alpha of protein phosphatase 1 (PPP1CA, hereafter referred to as PP1). Axin condensates promoted phosphorylation by enriching CK1α over PP1. Importantly, the phosphorylation took p","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2026-05-29T10:23:42.779Z","creation":"2025-04-04T11:02:33.729Z"},"accession":"S-EPMC10652037","cross_references":{"pubmed":["37721093"],"doi":["10.1242/jcs.261214"]}}