{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Agrawal A"],"funding":["National Institutes of Health","National Institute of General Medical Sciences"],"pagination":["e00228"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10652127"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17"],"pubmed_abstract":["Geraniol is a monoterpene with wide applications in the food, cosmetics, and pharmaceutical industries. Microbial production has largely used model organisms lacking favorable properties for monoterpene production. In this work, we produced geraniol in metabolically engineered <i>Yarrowia lipolytica</i>. First, two plant-derived geraniol synthases (GES) from <i>Catharanthus roseus</i> (Cr) and <i>Valeriana officinalis</i> (Vo) were tested based on previous reports of activity. Both wild type and truncated mutants of GES (without signal peptide targeting chloroplast) were examined by co-expressing with MVA pathway enzymes tHMG1 and IDI1. Truncated CrGES (tCrGES) produced the most geraniol and thus was used for further experimentation. The initial strain was obtained by overexpression of the"],"journal":["Metabolic engineering communications"],"pubmed_title":["Engineering <i>Yarrowia lipolytica</i> for the biosynthesis of geraniol."],"pmcid":["PMC10652127"],"funding_grant_id":["1R35GM133803"],"pubmed_authors":["Yang Z","Agrawal A","Blenner M"],"additional_accession":[]},"is_claimable":false,"name":"Engineering <i>Yarrowia lipolytica</i> for the biosynthesis of geraniol.","description":"Geraniol is a monoterpene with wide applications in the food, cosmetics, and pharmaceutical industries. Microbial production has largely used model organisms lacking favorable properties for monoterpene production. In this work, we produced geraniol in metabolically engineered <i>Yarrowia lipolytica</i>. First, two plant-derived geraniol synthases (GES) from <i>Catharanthus roseus</i> (Cr) and <i>Valeriana officinalis</i> (Vo) were tested based on previous reports of activity. Both wild type and truncated mutants of GES (without signal peptide targeting chloroplast) were examined by co-expressing with MVA pathway enzymes tHMG1 and IDI1. Truncated CrGES (tCrGES) produced the most geraniol and thus was used for further experimentation. The initial strain was obtained by overexpression of the","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2025-04-06T23:15:24.633Z","creation":"2025-04-06T23:15:24.633Z"},"accession":"S-EPMC10652127","cross_references":{"pubmed":["38029016"],"doi":["10.1016/j.mec.2023.e00228"]}}