biostudies-literaturePhilip AAHHS | National Institutes of HealthLawrence M. Blatt EndowmentGIVAX, INCe0060323https://www.ebi.ac.uk/biostudies/studies/S-EPMC10652971biostudies-literatureUnknown12(11)The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing improvements to its efficacy.Microbiology resource announcementsT7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix , GSK) vaccine strain.PMC10652971R21AI44881Patton JTYi GPhilip AAAgbemabiese CAfalseT7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix , GSK) vaccine strain.The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing improvements to its efficacy.2023-01-01T00:00:00Z2023 Nov2025-04-22T15:32:21.986Z2025-04-06T01:25:03.204ZS-EPMC106529713781912310.1128/MRA.00603-23