biostudies-literatureUnknown8(45)Kazakova EYellow fever virus (YFV) transmitted by infected mosquitoes causes an acute viral disease for which there are no approved small-molecule therapeutics. Our recently developed machine learning models for YFV inhibitors led to the selection of a new pyrazolesulfonamide derivative <b>RCB16003</b> with acceptable <i>in vitro</i> activity. We report that the <i>N</i>-phenyl-1-(phenylsulfonyl)-1<i>H</i>-1,2,4-triazol-3-amine class, which was recently identified as active non-nucleoside reverse transcriptase inhibitors against HIV-1, can also be repositioned as inhibitors of yellow fever virus replication. As compared to other <i>Flaviviridae</i> or <i>Togaviridae</i> family viruses tested, both compounds <b>RCB16003</b> and <b>RCB16007</b> demonstrate selectivity for YFV over related viruses, witACS omega42951-42965https://www.ebi.ac.uk/biostudies/studies/S-EPMC10653066biostudies-literature1-Sulfonyl-3-amino-1<i>H</i>-1,2,4-triazoles as Yellow Fever Virus Inhibitors: Synthesis and Structure-Activity Relationship.PMC10653066Riabova OEkins SKazakova EMakarov VPuhl ACLane TRJones Tfalse1-Sulfonyl-3-amino-1<i>H</i>-1,2,4-triazoles as Yellow Fever Virus Inhibitors: Synthesis and Structure-Activity Relationship.Yellow fever virus (YFV) transmitted by infected mosquitoes causes an acute viral disease for which there are no approved small-molecule therapeutics. Our recently developed machine learning models for YFV inhibitors led to the selection of a new pyrazolesulfonamide derivative <b>RCB16003</b> with acceptable <i>in vitro</i> activity. We report that the <i>N</i>-phenyl-1-(phenylsulfonyl)-1<i>H</i>-1,2,4-triazol-3-amine class, which was recently identified as active non-nucleoside reverse transcriptase inhibitors against HIV-1, can also be repositioned as inhibitors of yellow fever virus replication. As compared to other <i>Flaviviridae</i> or <i>Togaviridae</i> family viruses tested, both compounds <b>RCB16003</b> and <b>RCB16007</b> demonstrate selectivity for YFV over related viruses, wit2023-01-01T00:00:00Z2023 Nov2025-04-04T09:30:22.778Z2025-04-04T09:30:22.778ZS-EPMC106530663802473310.1021/acsomega.3c06106