biostudies-literatureUnknown64(14)Velez G<h4>Purpose</h4>The purpose of this study was to profile protein expression liquid vitreous biopsies from patients with uveal melanoma (UM) using mass spectrometry to identify prognostic biomarkers, signaling pathways, and therapeutic targets.<h4>Methods</h4>Vitreous biopsies were collected from two cohorts in a pilot study: comparative control eyes with epiretinal membranes (ERM; n = 3) and test eyes with UM (n = 8). Samples were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Identified proteins were compared to data from a targeted multiplex ELISA proteomics platform.<h4>Results</h4>A total of 69 significantly elevated proteins were detected in the UM vitreous, including LYVE-1. LC-MS/MS identified 62 significantly upregulated proteins in UM vitreous that were Investigative ophthalmology & visual science14https://www.ebi.ac.uk/biostudies/studies/S-EPMC10653261biostudies-literatureCross-Platform Identification and Validation of Uveal Melanoma Vitreous Protein Biomarkers.PMC10653261Dufour AMahajan VBWolf JVelez GMruthyunjaya PfalseCross-Platform Identification and Validation of Uveal Melanoma Vitreous Protein Biomarkers.<h4>Purpose</h4>The purpose of this study was to profile protein expression liquid vitreous biopsies from patients with uveal melanoma (UM) using mass spectrometry to identify prognostic biomarkers, signaling pathways, and therapeutic targets.<h4>Methods</h4>Vitreous biopsies were collected from two cohorts in a pilot study: comparative control eyes with epiretinal membranes (ERM; n = 3) and test eyes with UM (n = 8). Samples were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Identified proteins were compared to data from a targeted multiplex ELISA proteomics platform.<h4>Results</h4>A total of 69 significantly elevated proteins were detected in the UM vitreous, including LYVE-1. LC-MS/MS identified 62 significantly upregulated proteins in UM vitreous that were 2023-01-01T00:00:00Z2023 Nov2025-04-04T22:16:28.477Z2025-02-19T01:19:08.523ZS-EPMC106532613795561210.1167/iovs.64.14.14