biostudies-literatureUnknown18(11)van Leeuwen ALI<h4>Background</h4>The endothelial angiopoietin/Tie2 system is an important regulator of endothelial permeability and targeting Tie2 reduces hemorrhagic shock-induced organ edema in males. However, sexual dimorphism of the endothelium has not been taken into account. This study investigated whether there are sex-related differences in the endothelial angiopoietin/Tie2 system and edema formation.<h4>Methods</h4>Adult male and female heterozygous Tie2 knockout mice (Tie2+/-) and wild-type controls (Tie2+/+) were included (n = 9 per group). Renal and pulmonary injury were determined by wet/dry weight ratio and H&E staining of tissue sections. Protein levels were studied in plasma by ELISA and pulmonary and renal mRNA expression levels by RT-qPCR.<h4>Results</h4>In Tie2+/+ mice, females had hiPloS onee0293673https://www.ebi.ac.uk/biostudies/studies/S-EPMC10653528biostudies-literatureFemale sex protects against renal edema, but not lung edema, in mice with partial deletion of the endothelial barrier regulator Tie2 compared to male sex.PMC10653528Dekker NAMBeijer EIbelings Rvan Meurs MRoelofs JJTHvan den Brom CEvan Leeuwen ALIvan der Steen MRAMolema GfalseFemale sex protects against renal edema, but not lung edema, in mice with partial deletion of the endothelial barrier regulator Tie2 compared to male sex.<h4>Background</h4>The endothelial angiopoietin/Tie2 system is an important regulator of endothelial permeability and targeting Tie2 reduces hemorrhagic shock-induced organ edema in males. However, sexual dimorphism of the endothelium has not been taken into account. This study investigated whether there are sex-related differences in the endothelial angiopoietin/Tie2 system and edema formation.<h4>Methods</h4>Adult male and female heterozygous Tie2 knockout mice (Tie2+/-) and wild-type controls (Tie2+/+) were included (n = 9 per group). Renal and pulmonary injury were determined by wet/dry weight ratio and H&E staining of tissue sections. Protein levels were studied in plasma by ELISA and pulmonary and renal mRNA expression levels by RT-qPCR.<h4>Results</h4>In Tie2+/+ mice, females had hi2023-01-01T00:00:00Z20232026-03-31T11:39:26.471Z2025-02-19T01:19:03.661ZS-EPMC106535283797201110.1371/journal.pone.0293673