biostudies-literatureDali OANSESe202302237https://www.ebi.ac.uk/biostudies/studies/S-EPMC10654101biostudies-literatureUnknown7(2)Neonicotinoids are a widely used class of insecticides that are being applied in agricultural fields. We examined the capacity of a neonicotinoid, thiacloprid (<i>thia</i>), to induce transgenerational effects in male mice. Pregnant outbred Swiss female mice were exposed to <i>thia</i> at embryonic days E6.5-E15.5 using different doses. Testis sections were used for morphology analysis, ELISAs for testosterone level analysis, RT-qPCR and RNA-seq for gene expression analysis, MEDIP-seq and MEDIP-qPCR techniques for DNA methylation analysis, and Western blot for a protein analysis. The number of meiotic double-strand breaks and the number of incomplete synapsed chromosomes were higher in the <i>thia</i> 6-treated group of F3 males. Genome-wide analysis of DNA methylation in spermatozoa reveaLife science allianceTransgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposure.PMC10654101R20155NNLegoff LSmagulova FMerret PEDiba Lahmidi MPakdel FDali OKernanec PYHeitz CD'Cruz SfalseTransgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposure.Neonicotinoids are a widely used class of insecticides that are being applied in agricultural fields. We examined the capacity of a neonicotinoid, thiacloprid (<i>thia</i>), to induce transgenerational effects in male mice. Pregnant outbred Swiss female mice were exposed to <i>thia</i> at embryonic days E6.5-E15.5 using different doses. Testis sections were used for morphology analysis, ELISAs for testosterone level analysis, RT-qPCR and RNA-seq for gene expression analysis, MEDIP-seq and MEDIP-qPCR techniques for DNA methylation analysis, and Western blot for a protein analysis. The number of meiotic double-strand breaks and the number of incomplete synapsed chromosomes were higher in the <i>thia</i> 6-treated group of F3 males. Genome-wide analysis of DNA methylation in spermatozoa revea2024-01-01T00:00:00Z2024 Feb2026-05-29T00:14:24.077Z2025-04-06T02:38:38.175ZS-EPMC106541013797318810.26508/lsa.202302237