biostudies-literatureUnknown339Ji CCircular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA viruses are highly diverse and have a broad range of hosts. In this study, we report the detection of Bo-Circo-like virus AH20-1 in the feces of diarrheal cattle. The virus has a circular genome of 3,912 nucleotides, three major putative open reading frames, and encodes a Rep gene of 310 amino acids. We found that the virus is closely related to the Bo-Circo-like virus CH strain, which belongs to the novel Kirkoviridae family. Furthermore, we conducted a nationwide surveillance program and found that the virus is prevalent in China (23.6%, 205/868), with the BCLa subtype being the predominant strain. Our findings suggest that the virus can infect sheep, highlighting the potential for cross-species transmissiVirus research199204https://www.ebi.ac.uk/biostudies/studies/S-EPMC10654594biostudies-literatureCircular replication-associated protein-encoding single-stranded DNA virus with risk of spillover is widely prevalent in domestic animals in China.PMC10654594Gong FJi CPan ZZhang YFeng YZhang XYao HSun XfalseCircular replication-associated protein-encoding single-stranded DNA virus with risk of spillover is widely prevalent in domestic animals in China.Circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA viruses are highly diverse and have a broad range of hosts. In this study, we report the detection of Bo-Circo-like virus AH20-1 in the feces of diarrheal cattle. The virus has a circular genome of 3,912 nucleotides, three major putative open reading frames, and encodes a Rep gene of 310 amino acids. We found that the virus is closely related to the Bo-Circo-like virus CH strain, which belongs to the novel Kirkoviridae family. Furthermore, we conducted a nationwide surveillance program and found that the virus is prevalent in China (23.6%, 205/868), with the BCLa subtype being the predominant strain. Our findings suggest that the virus can infect sheep, highlighting the potential for cross-species transmissi2023-01-01T00:00:00Z2023 Nov2025-04-03T22:18:18.602Z2024-10-15T17:38:40.347ZS-EPMC106545943760759610.1016/j.virusres.2023.199204