biostudies-literatureVilla-Consuegra SMinisterio de Educación y Formación ProfesionalMinisterio de Ciencia e InnovaciónGobierno de Espana Ministerio de Ciencia e Innovacion108339https://www.ebi.ac.uk/biostudies/studies/S-EPMC10654595biostudies-literatureUnknown26(11)During meiosis, faithful chromosome segregation requires monopolar spindle microtubule-kinetochore arrays in MI to segregate homologous chromosomes, but bipolar in MII to segregate sister chromatids. Using fission yeasts, we found that the universal Aurora B kinase localizes to kinetochores in metaphase I and in the mid-spindle during anaphase I, as in mitosis; but in the absence of an intervening S phase, the importin α Imp1 propitiates its release from the spindle midzone to re-localize at kinetochores during meiotic interkinesis. We show that "error-correction" activity of kinetochore re-localized Aurora B becomes essential to erase monopolar arrangements from anaphase I, a prerequisite to satisfy the spindle assembly checkpoint (SAC) and to generate proper bipolar arrays at the onset oiScienceAurora B kinase erases monopolar microtubule-kinetochore arrays at the meiosis I-II transition.PMC10654595PID2019-111124GB-I00FPU18/04507Tallada VAVilla-Consuegra SJimenez JfalseAurora B kinase erases monopolar microtubule-kinetochore arrays at the meiosis I-II transition.During meiosis, faithful chromosome segregation requires monopolar spindle microtubule-kinetochore arrays in MI to segregate homologous chromosomes, but bipolar in MII to segregate sister chromatids. Using fission yeasts, we found that the universal Aurora B kinase localizes to kinetochores in metaphase I and in the mid-spindle during anaphase I, as in mitosis; but in the absence of an intervening S phase, the importin α Imp1 propitiates its release from the spindle midzone to re-localize at kinetochores during meiotic interkinesis. We show that "error-correction" activity of kinetochore re-localized Aurora B becomes essential to erase monopolar arrangements from anaphase I, a prerequisite to satisfy the spindle assembly checkpoint (SAC) and to generate proper bipolar arrays at the onset o2023-01-01T00:00:00Z2023 Nov2025-04-21T18:23:25.165Z2025-04-05T17:12:19.633ZS-EPMC106545953802618010.1016/j.isci.2023.108339