{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kaur P"],"funding":["NCI NIH HHS"],"pagination":["105296"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10656236"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["299(11)"],"pubmed_abstract":["3D chromatin organization plays a critical role in regulating gene expression, DNA replication, recombination, and repair. While initially discovered for its role in sister chromatid cohesion, emerging evidence suggests that the cohesin complex (SMC1, SMC3, RAD21, and SA1/SA2), facilitated by NIPBL, mediates topologically associating domains and chromatin loops through DNA loop extrusion. However, information on how conformational changes of cohesin-NIPBL drive its loading onto DNA, initiation, and growth of DNA loops is still lacking. In this study, high-speed atomic force microscopy imaging reveals that cohesin-NIPBL captures DNA through arm extension, assisted by feet (shorter protrusions), and followed by transfer of DNA to its lower compartment (SMC heads, RAD21, SA1, and NIPBL). Whil"],"journal":["The Journal of biological chemistry"],"pubmed_title":["High-speed AFM imaging reveals DNA capture and loop extrusion dynamics by cohesin-NIPBL."],"pmcid":["PMC10656236"],"funding_grant_id":["P01 CA092584"],"pubmed_authors":["Irvin EM","Lu X","Xu Q","Pappas C","Zhang H","Kaur P","Finkelstein IJ","Wang H","Shi Z","Yu H","Tao YJ"],"additional_accession":[]},"is_claimable":false,"name":"High-speed AFM imaging reveals DNA capture and loop extrusion dynamics by cohesin-NIPBL.","description":"3D chromatin organization plays a critical role in regulating gene expression, DNA replication, recombination, and repair. While initially discovered for its role in sister chromatid cohesion, emerging evidence suggests that the cohesin complex (SMC1, SMC3, RAD21, and SA1/SA2), facilitated by NIPBL, mediates topologically associating domains and chromatin loops through DNA loop extrusion. However, information on how conformational changes of cohesin-NIPBL drive its loading onto DNA, initiation, and growth of DNA loops is still lacking. In this study, high-speed atomic force microscopy imaging reveals that cohesin-NIPBL captures DNA through arm extension, assisted by feet (shorter protrusions), and followed by transfer of DNA to its lower compartment (SMC heads, RAD21, SA1, and NIPBL). Whil","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-21T23:55:04.402Z","creation":"2025-04-05T19:23:18.605Z"},"accession":"S-EPMC10656236","cross_references":{"pubmed":["37774974"],"doi":["10.1016/j.jbc.2023.105296"]}}