biostudies-literatureLiu YGuizhou Science and Technology DepartmentChina National Tobacco CorporationNational Natural Science Foundation of ChinaChina Tobacco Guizhou Industrial7543-7555https://www.ebi.ac.uk/biostudies/studies/S-EPMC10656326biostudies-literatureUnknown107(24)Rebuilding soil healthy microbiota is very important for preventing bacterial wilt. A 3-year-long field trial was conducted in China as follows: T1 (conventional fertilization), T2 (T1 + liming), T3 (T1 + bioorganic fertilizer), and T4 (T2 + bioorganic fertilizer). Fluorescence quantitative PCR and high-throughput sequencing were employed to study the dynamics of Ralstonia solanacearum population, microbial community, and network organizations between bacteria and quality-related variables. After 3 years of bioremediation, the control efficacy of tobacco bacterial wilt reached 61.30% and the occurrence delayed by approximately 40 days in T4, which had the highest tobacco yield and output value. The pathogen population of T4 remained below 10⁶ copies/g soil during the entire growApplied microbiology and biotechnologyEffect of soil bioremediation on soil microbial community structure aimed at controlling tobacco bacterial wilt.PMC10656326318605971102022020152019052022XM032021XM18[2020]4004Liu YLi HLi XPeng YZhu JZhang HSun GXu JfalseEffect of soil bioremediation on soil microbial community structure aimed at controlling tobacco bacterial wilt.Rebuilding soil healthy microbiota is very important for preventing bacterial wilt. A 3-year-long field trial was conducted in China as follows: T1 (conventional fertilization), T2 (T1 + liming), T3 (T1 + bioorganic fertilizer), and T4 (T2 + bioorganic fertilizer). Fluorescence quantitative PCR and high-throughput sequencing were employed to study the dynamics of Ralstonia solanacearum population, microbial community, and network organizations between bacteria and quality-related variables. After 3 years of bioremediation, the control efficacy of tobacco bacterial wilt reached 61.30% and the occurrence delayed by approximately 40 days in T4, which had the highest tobacco yield and output value. The pathogen population of T4 remained below 10⁶ copies/g soil during the entire grow2023-01-01T00:00:00Z2023 Dec2026-07-14T15:36:19.794Z2025-04-07T05:49:25.041ZS-EPMC106563263775551110.1007/s00253-023-12753-4