{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Benavente R"],"funding":["NIA NIH HHS","NIAID NIH HHS"],"pagination":["20171"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10656533"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Chronic wasting disease (CWD) is a prion disease affecting cervids. CWD diagnosis is conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD-prions). Two in vitro prion amplification techniques, real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA), have shown promise in detecting CWD-prions in tissues and bodily fluids. Recent studies have demonstrated that RT-QuIC yields similar results compared to ELISA and IHC. Here, we analyzed 1003 retropharyngeal lymph nodes (RPLNs) from Texas white-tailed deer. PMCA detected CWD at a higher rate compared to ELISA/IHC, identified different prion strains, and"],"journal":["Scientific reports"],"pubmed_title":["PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC."],"pmcid":["PMC10656533"],"funding_grant_id":["RF1 AG072491","R01 AI132695"],"pubmed_authors":["Reed JH","Benavente R","Lockwood M","Morales R"],"additional_accession":[]},"is_claimable":false,"name":"PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC.","description":"Chronic wasting disease (CWD) is a prion disease affecting cervids. CWD diagnosis is conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD-prions). Two in vitro prion amplification techniques, real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA), have shown promise in detecting CWD-prions in tissues and bodily fluids. Recent studies have demonstrated that RT-QuIC yields similar results compared to ELISA and IHC. Here, we analyzed 1003 retropharyngeal lymph nodes (RPLNs) from Texas white-tailed deer. PMCA detected CWD at a higher rate compared to ELISA/IHC, identified different prion strains, and","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-06-26T03:24:30.897Z","creation":"2025-04-05T18:35:47.549Z"},"accession":"S-EPMC10656533","cross_references":{"pubmed":["37978312"],"doi":["10.1038/s41598-023-47105-9"]}}