{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Xu S"],"pubmed_abstract":["Single-nucleotide polymorphism (SNP) plays a key role in the carcinogenesis of the human genome, and understanding the intrinsic relationship between individual genetic variations and carcinogenesis lies heavily in the establishment of a precise and sensitive SNP detection platform. Given this, a powerful and reliable SNP detection platform is proposed by a flap endonuclease 1 (FEN 1)-driven DNA walker-like reaction coupling with a magnetic bead (MB)-based separation. A carboxyfluorescein (FAM)-labeled downstream probe (DP) was decorated on a streptavidin magnetic bead (SMB). The target DNA, as a walker strand, was captured by hybridization with DP and an upstream probe (UP) to form a three-base overlapping structure and execute the walking function on the surface of SMB. FEN 1 was employe"],"journal":["Frontiers in bioengineering and biotechnology"],"pagination":["1279473"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10656677"],"repository":["biostudies-literature"],"pubmed_title":["A FEN 1-driven DNA walker-like reaction coupling with magnetic bead-based separation for specific SNP detection."],"pmcid":["PMC10656677"],"pubmed_authors":["Yang Z","Liu L","Chen J","Bian L","Zhang Y","Xu S","Yang F","Xu J","Wang L","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"A FEN 1-driven DNA walker-like reaction coupling with magnetic bead-based separation for specific SNP detection.","description":"Single-nucleotide polymorphism (SNP) plays a key role in the carcinogenesis of the human genome, and understanding the intrinsic relationship between individual genetic variations and carcinogenesis lies heavily in the establishment of a precise and sensitive SNP detection platform. Given this, a powerful and reliable SNP detection platform is proposed by a flap endonuclease 1 (FEN 1)-driven DNA walker-like reaction coupling with a magnetic bead (MB)-based separation. A carboxyfluorescein (FAM)-labeled downstream probe (DP) was decorated on a streptavidin magnetic bead (SMB). The target DNA, as a walker strand, was captured by hybridization with DP and an upstream probe (UP) to form a three-base overlapping structure and execute the walking function on the surface of SMB. FEN 1 was employe","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-22T18:06:47.168Z","creation":"2025-04-06T02:21:18.379Z"},"accession":"S-EPMC10656677","cross_references":{"pubmed":["38026850"],"doi":["10.3389/fbioe.2023.1279473"]}}