biostudies-literatureUnknown11Xu SSingle-nucleotide polymorphism (SNP) plays a key role in the carcinogenesis of the human genome, and understanding the intrinsic relationship between individual genetic variations and carcinogenesis lies heavily in the establishment of a precise and sensitive SNP detection platform. Given this, a powerful and reliable SNP detection platform is proposed by a flap endonuclease 1 (FEN 1)-driven DNA walker-like reaction coupling with a magnetic bead (MB)-based separation. A carboxyfluorescein (FAM)-labeled downstream probe (DP) was decorated on a streptavidin magnetic bead (SMB). The target DNA, as a walker strand, was captured by hybridization with DP and an upstream probe (UP) to form a three-base overlapping structure and execute the walking function on the surface of SMB. FEN 1 was employeFrontiers in bioengineering and biotechnology1279473https://www.ebi.ac.uk/biostudies/studies/S-EPMC10656677biostudies-literatureA FEN 1-driven DNA walker-like reaction coupling with magnetic bead-based separation for specific SNP detection.PMC10656677Yang ZLiu LChen JBian LZhang YXu SYang FXu JWang LZhao XfalseA FEN 1-driven DNA walker-like reaction coupling with magnetic bead-based separation for specific SNP detection.Single-nucleotide polymorphism (SNP) plays a key role in the carcinogenesis of the human genome, and understanding the intrinsic relationship between individual genetic variations and carcinogenesis lies heavily in the establishment of a precise and sensitive SNP detection platform. Given this, a powerful and reliable SNP detection platform is proposed by a flap endonuclease 1 (FEN 1)-driven DNA walker-like reaction coupling with a magnetic bead (MB)-based separation. A carboxyfluorescein (FAM)-labeled downstream probe (DP) was decorated on a streptavidin magnetic bead (SMB). The target DNA, as a walker strand, was captured by hybridization with DP and an upstream probe (UP) to form a three-base overlapping structure and execute the walking function on the surface of SMB. FEN 1 was employe2023-01-01T00:00:00Z20232025-04-22T18:06:47.168Z2025-04-06T02:21:18.379ZS-EPMC106566773802685010.3389/fbioe.2023.1279473