biostudies-literatureSchneider KHÖsterreichische Wissenschaftsfonds (AT)117https://www.ebi.ac.uk/biostudies/studies/S-EPMC10656895biostudies-literatureUnknown27(1)<h4>Background</h4>There is a great clinical need and it remains a challenge to develop artificial soft tissue constructs that can mimic the biomechanical properties and bioactivity of natural tissue. This is partly due to the lack of suitable biomaterials. Hydrogels made from human placenta offer high bioactivity and represent a potential solution to create animal-free 3D bioprinting systems that are both sustainable and acceptable, as placenta is widely considered medical waste. A combination with silk and gelatin polymers can bridge the biomechanical limitations of human placenta chorion extracellular matrix hydrogels (hpcECM) while maintaining their excellent bioactivity.<h4>Method</h4>In this study, silk fibroin (SF) and tyramine-substituted gelatin (G-TA) were enzymatically crosslinkBiomaterials researchSilk fibroin, gelatin, and human placenta extracellular matrix-based composite hydrogels for 3D bioprinting and soft tissue engineering.PMC10656895J4491-BMu XKaplan DLDotzlhofer MTheodossiou SFitzpatrick VTeuschl-Woller AHRiess PRohringer SSchneider KHPodesser BKHasturk OPichelkastner LEnayati MBergmeister HGoldberg BJEder GKiss HfalseSilk fibroin, gelatin, and human placenta extracellular matrix-based composite hydrogels for 3D bioprinting and soft tissue engineering.<h4>Background</h4>There is a great clinical need and it remains a challenge to develop artificial soft tissue constructs that can mimic the biomechanical properties and bioactivity of natural tissue. This is partly due to the lack of suitable biomaterials. Hydrogels made from human placenta offer high bioactivity and represent a potential solution to create animal-free 3D bioprinting systems that are both sustainable and acceptable, as placenta is widely considered medical waste. A combination with silk and gelatin polymers can bridge the biomechanical limitations of human placenta chorion extracellular matrix hydrogels (hpcECM) while maintaining their excellent bioactivity.<h4>Method</h4>In this study, silk fibroin (SF) and tyramine-substituted gelatin (G-TA) were enzymatically crosslink2023-01-01T00:00:00Z2023 Nov2025-04-21T22:15:28.803Z2025-02-18T23:29:54.944ZS-EPMC106568953797839910.1186/s40824-023-00431-5