{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Moon J"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","U.S. Department of Health & Human Services | NIH | National Cancer Institute","NCI NIH HHS"],"pagination":["7504"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10657364"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Nucleic acid detection powered by CRISPR technology provides a rapid, sensitive, and deployable approach to molecular diagnostics. While exciting, there remain challenges limiting its practical applications, such as the need for pre-amplification and the lack of quantitative ability. Here, we develop an asymmetric CRISPR assay for cascade signal amplification detection of nucleic acids by leveraging the asymmetric trans-cleavage behavior of competitive crRNA. We discover that the competitive reaction between a full-sized crRNA and split crRNA for CRISPR-Cas12a can induce cascade signal amplification, significantly improving the target detection signal. In addition, we find that CRISPR-Cas12a can recognize fragmented RNA/DNA targets, enabling direct RNA detection by Cas12a. Based on these f"],"journal":["Nature communications"],"pubmed_title":["Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA."],"pmcid":["PMC10657364"],"funding_grant_id":["U01CA269147","U01 CA269147","U01AI148306","U01 AI148306"],"pubmed_authors":["Liu C","Moon J"],"additional_accession":[]},"is_claimable":false,"name":"Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA.","description":"Nucleic acid detection powered by CRISPR technology provides a rapid, sensitive, and deployable approach to molecular diagnostics. While exciting, there remain challenges limiting its practical applications, such as the need for pre-amplification and the lack of quantitative ability. Here, we develop an asymmetric CRISPR assay for cascade signal amplification detection of nucleic acids by leveraging the asymmetric trans-cleavage behavior of competitive crRNA. We discover that the competitive reaction between a full-sized crRNA and split crRNA for CRISPR-Cas12a can induce cascade signal amplification, significantly improving the target detection signal. In addition, we find that CRISPR-Cas12a can recognize fragmented RNA/DNA targets, enabling direct RNA detection by Cas12a. Based on these f","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-19T06:36:05.302Z","creation":"2025-04-19T06:36:05.302Z"},"accession":"S-EPMC10657364","cross_references":{"pubmed":["37980404"],"doi":["10.1038/s41467-023-43389-7"]}}