biostudies-literatureSever RENCI NIH HHSNational Institutes of HealthNIH HHS20214https://www.ebi.ac.uk/biostudies/studies/S-EPMC10657433biostudies-literatureUnknown13(1)Neuroblastoma accounts for 15% of pediatric cancer deaths, despite multimodal therapy including surgical resection. Current neuroblastoma rodent models are insufficient for studying the impact of surgery and combination treatments, largely due to the small size of mouse models. Human neuroblastoma SK-N-BE(2) cells were injected into the left adrenal gland of 5-6-week-old RNU homozygous nude rats. Rats were either monitored by MRI until humane endpoint was reached or after 5 weeks underwent operative tumor resection, followed by monitoring for recurrence and survival. Following neuroblastoma cell implantation, the majority of tumors grew to greater than 5000 mm³ within 5.5-6.5 weeks, meeting the humane endpoint. Surgical resection was successfully done in 8 out of 9 rats, extendiScientific reportsSurvival following complete resection of neuroblastoma in novel orthotopic rat xenograft model.PMC10657433R01 CA277664T32CA113263T32 CA113263Malek MMKohanbash GReyes-Mugica MSever RERosenblum LTEdwards WBfalseSurvival following complete resection of neuroblastoma in novel orthotopic rat xenograft model.Neuroblastoma accounts for 15% of pediatric cancer deaths, despite multimodal therapy including surgical resection. Current neuroblastoma rodent models are insufficient for studying the impact of surgery and combination treatments, largely due to the small size of mouse models. Human neuroblastoma SK-N-BE(2) cells were injected into the left adrenal gland of 5-6-week-old RNU homozygous nude rats. Rats were either monitored by MRI until humane endpoint was reached or after 5 weeks underwent operative tumor resection, followed by monitoring for recurrence and survival. Following neuroblastoma cell implantation, the majority of tumors grew to greater than 5000 mm³ within 5.5-6.5 weeks, meeting the humane endpoint. Surgical resection was successfully done in 8 out of 9 rats, extendi2023-01-01T00:00:00Z2023 Nov2026-07-14T16:48:43.888Z2025-04-06T22:46:27.517ZS-EPMC106574333798038810.1038/s41598-023-47537-3