biostudies-literatureJiajia Hthe National Natural Science Foundation of China567https://www.ebi.ac.uk/biostudies/studies/S-EPMC10657630biostudies-literatureUnknown23(1)UFMylation is a ubiquitination-like modification that is related to endoplasmic reticulum stress and unfolded protein response. A recent study reported that Ufl1, a key enzyme of UFMylation, protects against heart failure, indicating that UFMylation may be associated with heart function regulation. In the present study, we initially constructed a Flag-6×His-tagged Ufm1ΔSC transgenic (Tg-Ufm1) mouse model that enables UFMylation studies in vivo. Tg-Ufm1 mice showed significant activation of UFMylation in hearts. By using this model, we identified 38 potential Ufm1-binding proteins in heart tissues through LC‒MS/MS methods. We found that these proteins were associated with mitochondria, metabolism and chaperone binding. By using transcriptomic screening, we identified Tnfaip2 as a novel UFMyBMC cardiovascular disordersScreening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice.PMC10657630No. 81870196Jiaqi ZZiyao YXiaotao ZYanli CMing SJiajia HfalseScreening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice.UFMylation is a ubiquitination-like modification that is related to endoplasmic reticulum stress and unfolded protein response. A recent study reported that Ufl1, a key enzyme of UFMylation, protects against heart failure, indicating that UFMylation may be associated with heart function regulation. In the present study, we initially constructed a Flag-6×His-tagged Ufm1ΔSC transgenic (Tg-Ufm1) mouse model that enables UFMylation studies in vivo. Tg-Ufm1 mice showed significant activation of UFMylation in hearts. By using this model, we identified 38 potential Ufm1-binding proteins in heart tissues through LC‒MS/MS methods. We found that these proteins were associated with mitochondria, metabolism and chaperone binding. By using transcriptomic screening, we identified Tnfaip2 as a novel UFMy2023-01-01T00:00:00Z2023 Nov2025-04-18T13:13:13.2Z2025-02-19T01:52:16.922ZS-EPMC106576303798050710.1186/s12872-023-03563-7