biostudies-literatureUnknown11Zhou Z<b>Background:</b> Currently, the mechanism(s) underlying corticogenesis is still under characterization. <b>Methods:</b> We curated the most comprehensive single-cell RNA-seq (scRNA-seq) datasets from mouse and human fetal cortexes for data analysis and confirmed the findings with co-immunostaining experiments. <b>Results:</b> By analyzing the developmental trajectories with scRNA-seq datasets in mice, we identified a specific developmental sub-path contributed by a cell-population expressing both deep- and upper-layer neurons (DLNs and ULNs) specific markers, which occurred on E13.5 but was absent in adults. In this cell-population, the percentages of cells expressing DLN and ULN markers decreased and increased, respectively, during the development suggesting direct neuronal transition (Frontiers in cell and developmental biology1209320https://www.ebi.ac.uk/biostudies/studies/S-EPMC10657809biostudies-literatureSingle-cell analysis reveals specific neuronal transition during mouse corticogenesis.PMC10657809Sun HXPan YZhang DLi JGu SZhou SDong ZZhou ZZhao LWang SZhu LCao YJiang XLin GfalseSingle-cell analysis reveals specific neuronal transition during mouse corticogenesis.<b>Background:</b> Currently, the mechanism(s) underlying corticogenesis is still under characterization. <b>Methods:</b> We curated the most comprehensive single-cell RNA-seq (scRNA-seq) datasets from mouse and human fetal cortexes for data analysis and confirmed the findings with co-immunostaining experiments. <b>Results:</b> By analyzing the developmental trajectories with scRNA-seq datasets in mice, we identified a specific developmental sub-path contributed by a cell-population expressing both deep- and upper-layer neurons (DLNs and ULNs) specific markers, which occurred on E13.5 but was absent in adults. In this cell-population, the percentages of cells expressing DLN and ULN markers decreased and increased, respectively, during the development suggesting direct neuronal transition (2023-01-01T00:00:00Z20232026-05-29T03:11:22.237Z2025-04-20T01:04:21.142ZS-EPMC106578093802090710.3389/fcell.2023.1209320