{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["72(4)"],"submitter":["Matsuo H"],"pubmed_abstract":["Hypertension and atherosclerosis are often found in one patient causing serious cardiovascular events. An animal model simultaneously expressing hypertension and atherosclerosis would be useful to study such a complex risk status. We therefore attempted to introduce a null mutation of the apolipoprotein E (ApoE) gene into the spontaneously hypertensive rat (SHR) using CRISPR/Cas9 to establish a genetic model for atherosclerosis with hypertension. We successfully established SHR<sup>ApoE(-/-)</sup> having a 13-bps deletion in the 5'-end of ApoE gene. Deletion of ApoE protein was confirmed by Western blotting. Blood pressure of SHR<sup>ApoE(-/-)</sup> was comparable to that of SHR. Feeding the rats with high fat high cholesterol diet (HFD) caused a significant increase in LDL cholesterol as "],"journal":["Experimental animals"],"pagination":["439-445"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10658095"],"repository":["biostudies-literature"],"pubmed_title":["Apolipoprotein E-depletion accelerates arterial fat deposition in the spontaneously hypertensive rat."],"pmcid":["PMC10658095"],"pubmed_authors":["Mashimo T","Matsuo H","Kawakami K","Yamada T","Nabika T","Kaneko T","Ohara H"],"additional_accession":[]},"is_claimable":false,"name":"Apolipoprotein E-depletion accelerates arterial fat deposition in the spontaneously hypertensive rat.","description":"Hypertension and atherosclerosis are often found in one patient causing serious cardiovascular events. An animal model simultaneously expressing hypertension and atherosclerosis would be useful to study such a complex risk status. We therefore attempted to introduce a null mutation of the apolipoprotein E (ApoE) gene into the spontaneously hypertensive rat (SHR) using CRISPR/Cas9 to establish a genetic model for atherosclerosis with hypertension. We successfully established SHR<sup>ApoE(-/-)</sup> having a 13-bps deletion in the 5'-end of ApoE gene. Deletion of ApoE protein was confirmed by Western blotting. Blood pressure of SHR<sup>ApoE(-/-)</sup> was comparable to that of SHR. Feeding the rats with high fat high cholesterol diet (HFD) caused a significant increase in LDL cholesterol as ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-04T12:18:11.459Z","creation":"2025-04-04T12:18:11.459Z"},"accession":"S-EPMC10658095","cross_references":{"pubmed":["37081644"],"doi":["10.1538/expanim.23-0012"]}}