biostudies-literatureUnknown53Wei W<h4>Introductions</h4>Ethylene regulates ripening by activating various metabolic pathways that controlcolor, aroma, flavor, texture, and consequently, the quality of fruits. However, the modulation of ethylene biosynthesis and quality formation during banana fruit ripening remains unclear.<h4>Objectives</h4>The present study aimed to identify the regulatory module that regulates ethylene and fruit quality-related metabolisms during banana fruit ripening.<h4>Methods</h4>We used RNA-seq to compare unripe and ripe banana fruits and identified a ripening-induced NAC transcription factor, MaNAC029. We further performed DNA affinity purification sequencing to identify the MaNAC029's target genes involved in ethylene biosynthesis and fruit quality formation, and electrophoretic mobility shift asJournal of advanced research33-47https://www.ebi.ac.uk/biostudies/studies/S-EPMC10658243biostudies-literatureMaNAC029 modulates ethylene biosynthesis and fruit quality and undergoes MaXB3-mediated proteasomal degradation during banana ripening.PMC10658243Shan WYang YYLakshmanan PKuang JFChen JYWei WLu WJfalseMaNAC029 modulates ethylene biosynthesis and fruit quality and undergoes MaXB3-mediated proteasomal degradation during banana ripening.<h4>Introductions</h4>Ethylene regulates ripening by activating various metabolic pathways that controlcolor, aroma, flavor, texture, and consequently, the quality of fruits. However, the modulation of ethylene biosynthesis and quality formation during banana fruit ripening remains unclear.<h4>Objectives</h4>The present study aimed to identify the regulatory module that regulates ethylene and fruit quality-related metabolisms during banana fruit ripening.<h4>Methods</h4>We used RNA-seq to compare unripe and ripe banana fruits and identified a ripening-induced NAC transcription factor, MaNAC029. We further performed DNA affinity purification sequencing to identify the MaNAC029's target genes involved in ethylene biosynthesis and fruit quality formation, and electrophoretic mobility shift as2023-01-01T00:00:00Z2023 Nov2025-04-22T09:24:39.435Z2025-04-05T23:03:49.635ZS-EPMC106582433652935110.1016/j.jare.2022.12.004