{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fan X"],"funding":["Jilin University","China Postdoctoral Science Foundation","National Natural Science Foundation of China","Natural Science Foundation of Jilin Province","Department of Science and Technology of Jilin Province"],"pagination":["561-567"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10658301"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(6)"],"pubmed_abstract":["<h4>Objective</h4>Baicalin, which is a key bioactive constituent obtained from <i>Scutellaria baicalensis</i>, has been utilized in traditional Chinese medicine for many centuries. Although it has been reported that Baicalin (BA) can inhibit the replication of the Hepatitis B virus (HBV), the exact mechanism behind this process remains unclear. Interferon-stimulated genes (ISGs) are crucial in the process of antiviral defense. We aim to investigate whether BA can regulate the expression of ISGs, and thereby potentially modulate the replication of HBV.<h4>Methods</h4>The study involved the use of CRISPR/Cas9 technology to perform knockout experiments on TRIM25 and IFIT3 genes. The expression of these genes was confirmed through techniques such as immunoblotting or Q-PCR. The levels of HBsAg"],"journal":["Journal of traditional and complementary medicine"],"pubmed_title":["Baicalin inhibits the replication of the hepatitis B virus by targeting TRIM25."],"pmcid":["PMC10658301"],"funding_grant_id":["2018B24","20220402085","2020M680044","20200201340JC","81801563","20210101338JC","81901592"],"pubmed_authors":["Li X","Fan X","Wei Q","Tan G","Wang F","Xu F","Song H","Lei B","Wang Y","Wang Z"],"additional_accession":[]},"is_claimable":false,"name":"Baicalin inhibits the replication of the hepatitis B virus by targeting TRIM25.","description":"<h4>Objective</h4>Baicalin, which is a key bioactive constituent obtained from <i>Scutellaria baicalensis</i>, has been utilized in traditional Chinese medicine for many centuries. Although it has been reported that Baicalin (BA) can inhibit the replication of the Hepatitis B virus (HBV), the exact mechanism behind this process remains unclear. Interferon-stimulated genes (ISGs) are crucial in the process of antiviral defense. We aim to investigate whether BA can regulate the expression of ISGs, and thereby potentially modulate the replication of HBV.<h4>Methods</h4>The study involved the use of CRISPR/Cas9 technology to perform knockout experiments on TRIM25 and IFIT3 genes. The expression of these genes was confirmed through techniques such as immunoblotting or Q-PCR. The levels of HBsAg","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-06-03T12:58:54.932Z","creation":"2025-04-05T00:24:38.811Z"},"accession":"S-EPMC10658301","cross_references":{"pubmed":["38020548"],"doi":["10.1016/j.jtcme.2023.05.009"]}}