biostudies-literatureUnknown53Zhang WShenzhen Science and Technology Innovation Commission<h4>Introduction</h4>Advanced paternal age of reproduction is an increasing trend, especially in developed countries and areas. This trend results in elevated risks of adverse reproductive outcomes such as reduced fertility rates, increased pregnancy loss, and poor childhood health. Yet, a systematic profiling of aging-associated molecular and cellular alterations in testicular tissue is still missing.<h4>Objectives</h4>We aimed to dissect aging-associated molecular characteristics in testes of mice.<h4>Methods</h4>Single-cell transcriptomic sequencing and analysis were conducted in testes of young (2 months old) and old mice (24 months old). Immunofluorescences and immunochemistry were used to characterize aging-associated phenotypes and verify single cell sequence results.<h4>Results</h4Journal of advanced research219-234https://www.ebi.ac.uk/biostudies/studies/S-EPMC10658307biostudies-literatureA single-cell transcriptomic landscape of mouse testicular aging.PMC10658307Yang JLiu JCao MXiao WXu CXia SYang CWang JZhang WTang LLi ZZhao SWang SSong YfalseA single-cell transcriptomic landscape of mouse testicular aging.<h4>Introduction</h4>Advanced paternal age of reproduction is an increasing trend, especially in developed countries and areas. This trend results in elevated risks of adverse reproductive outcomes such as reduced fertility rates, increased pregnancy loss, and poor childhood health. Yet, a systematic profiling of aging-associated molecular and cellular alterations in testicular tissue is still missing.<h4>Objectives</h4>We aimed to dissect aging-associated molecular characteristics in testes of mice.<h4>Methods</h4>Single-cell transcriptomic sequencing and analysis were conducted in testes of young (2 months old) and old mice (24 months old). Immunofluorescences and immunochemistry were used to characterize aging-associated phenotypes and verify single cell sequence results.<h4>Results</h42023-01-01T00:00:00Z2023 Nov2025-04-22T09:30:49.568Z2025-04-05T23:03:32.665ZS-EPMC106583073652829410.1016/j.jare.2022.12.007