biostudies-literatureUnknownAlvarez-Kuglen MNIA NIH HHSNCI NIH HHSBiomarkers of biological age that predict the risk of disease and expected lifespan better than chronological age are key to efficient and cost-effective healthcare^1-3. To advance a personalized approach to healthcare, such biomarkers must reliably and accurately capture individual biology, predict biological age, and provide scalable and cost-effective measurements. We developed a novel approach - image-based chromatin and epigenetic age (ImAge) that captures intrinsic progressions of biological age, which readily emerge as principal changes in the spatial organization of chromatin and epigenetic marks in single nuclei without regression on chronological age. ImAge captured the expected acceleration or deceleration of biological age in mice treated with chemotherapy or followinResearch squarers.3.rs-3479973https://www.ebi.ac.uk/biostudies/studies/S-EPMC10659560biostudies-literatureImaging-based chromatin and epigenetic age, ImAge, quantitates aging and rejuvenation.PMC10659560R21 AG075483P30 CA030199R21 AG068913Sharpee TORoberts AJHavas AAdams PDTerskikh AVRodriguez DAnderson RMHsu WMAlvarez-Kuglen MNinomiya KQin HFiengo LFarhy CSerrano MFeng GSfalseImaging-based chromatin and epigenetic age, ImAge, quantitates aging and rejuvenation.Biomarkers of biological age that predict the risk of disease and expected lifespan better than chronological age are key to efficient and cost-effective healthcare^1-3. To advance a personalized approach to healthcare, such biomarkers must reliably and accurately capture individual biology, predict biological age, and provide scalable and cost-effective measurements. We developed a novel approach - image-based chromatin and epigenetic age (ImAge) that captures intrinsic progressions of biological age, which readily emerge as principal changes in the spatial organization of chromatin and epigenetic marks in single nuclei without regression on chronological age. ImAge captured the expected acceleration or deceleration of biological age in mice treated with chemotherapy or followin2023-01-01T00:00:00Z2023 Nov2025-04-22T15:37:52.943Z2025-04-06T01:23:38.707ZS-EPMC106595603798694710.21203/rs.3.rs-3479973/v1