{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Canuto F"],"funding":["Conseil Régional Aquitaine"],"pagination":["1289100"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10662966"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["<h4>Introduction</h4>The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we identified <i>Euscelidius variegatus</i> cell proteins interacting with recombinant VmpA-His<sub>6</sub>.<h4>Methods</h4>The <i>E. variegatus</i> proteins were identified by mass spectrometry analysis of VmpA-<i>E. variegatus</i> protein complexes formed upon <i>in vitro</i> interaction assays. To assess their impact in VmpA binding, we reduced the expression of the candidate genes on <i>E. variegatus</i> cells in culture by dsRNA-mediated RNAi. The effect of candidate gene knockdown on VmpA binding was measured"],"journal":["Frontiers in cellular and infection microbiology"],"pubmed_title":["A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence doree phytoplasma."],"pmcid":["PMC10662966"],"funding_grant_id":["Grant AAPR2020A-2019-8404510/810"],"pubmed_authors":["Canuto F","Dubrana MP","Arricau-Bouvery N","Duret S","Claverol S","Malembic-Maher S","Foissac X"],"additional_accession":[]},"is_claimable":false,"name":"A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence doree phytoplasma.","description":"<h4>Introduction</h4>The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we identified <i>Euscelidius variegatus</i> cell proteins interacting with recombinant VmpA-His<sub>6</sub>.<h4>Methods</h4>The <i>E. variegatus</i> proteins were identified by mass spectrometry analysis of VmpA-<i>E. variegatus</i> protein complexes formed upon <i>in vitro</i> interaction assays. To assess their impact in VmpA binding, we reduced the expression of the candidate genes on <i>E. variegatus</i> cells in culture by dsRNA-mediated RNAi. The effect of candidate gene knockdown on VmpA binding was measured","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-06-17T06:40:49.568Z","creation":"2025-04-04T09:51:23.368Z"},"accession":"S-EPMC10662966","cross_references":{"pubmed":["38029232"],"doi":["10.3389/fcimb.2023.1289100"]}}