biostudies-literatureCanuto FConseil Régional Aquitaine1289100https://www.ebi.ac.uk/biostudies/studies/S-EPMC10662966biostudies-literatureUnknown13<h4>Introduction</h4>The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we identified <i>Euscelidius variegatus</i> cell proteins interacting with recombinant VmpA-His₆.<h4>Methods</h4>The <i>E. variegatus</i> proteins were identified by mass spectrometry analysis of VmpA-<i>E. variegatus</i> protein complexes formed upon <i>in vitro</i> interaction assays. To assess their impact in VmpA binding, we reduced the expression of the candidate genes on <i>E. variegatus</i> cells in culture by dsRNA-mediated RNAi. The effect of candidate gene knockdown on VmpA binding was measuredFrontiers in cellular and infection microbiologyA knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence doree phytoplasma.PMC10662966Grant AAPR2020A-2019-8404510/810Canuto FDubrana MPArricau-Bouvery NDuret SClaverol SMalembic-Maher SFoissac XfalseA knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence doree phytoplasma.<h4>Introduction</h4>The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we identified <i>Euscelidius variegatus</i> cell proteins interacting with recombinant VmpA-His₆.<h4>Methods</h4>The <i>E. variegatus</i> proteins were identified by mass spectrometry analysis of VmpA-<i>E. variegatus</i> protein complexes formed upon <i>in vitro</i> interaction assays. To assess their impact in VmpA binding, we reduced the expression of the candidate genes on <i>E. variegatus</i> cells in culture by dsRNA-mediated RNAi. The effect of candidate gene knockdown on VmpA binding was measured2023-01-01T00:00:00Z20232026-06-17T06:40:49.568Z2025-04-04T09:51:23.368ZS-EPMC106629663802923210.3389/fcimb.2023.1289100