{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang P"],"funding":["National Institute of Environmental Health Sciences","American Cancer Society","NIEHS NIH HHS"],"pagination":["e9095"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10668917"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(13)"],"pubmed_abstract":["Rationale
As a new approach to DNA adductomics, we directly reacted intact, double-stranded (ds)-DNA under warm conditions with an alkylating mass tag followed by analysis by liquid chromatography/mass spectrometry. This method is based on the tendency of adducted nucleobases to locally disrupt the DNA structure (forming a \"DNA bubble\") potentially increasing exposure of their nucleophilic (including active hydrogen) sites for preferential alkylation. Also encouraging this strategy is that the scope of nucleotide excision repair is very broad, and this system primarily recognizes DNA bubbles.Methods
A cationic xylyl (CAX) mass tag with limited nonpolarity was selected to increase the retention of polar adducts in reversed-phase high-performance liquid chromatography (HPLC) "],"journal":["Rapid communications in mass spectrometry : RCM"],"pubmed_title":["DNA Adductomics by mass tag prelabeling."],"pmcid":["PMC10668917"],"funding_grant_id":["P42 ES017198","P42ES017198","RSG‐12‐161‐01‐DMC"],"pubmed_authors":["Kramer CS","Wang P","Beuning PJ","Walsh CA","Roider E","Coulter ME","Giese RW"],"additional_accession":[]},"is_claimable":false,"name":"DNA Adductomics by mass tag prelabeling.","description":"Rationale
As a new approach to DNA adductomics, we directly reacted intact, double-stranded (ds)-DNA under warm conditions with an alkylating mass tag followed by analysis by liquid chromatography/mass spectrometry. This method is based on the tendency of adducted nucleobases to locally disrupt the DNA structure (forming a \"DNA bubble\") potentially increasing exposure of their nucleophilic (including active hydrogen) sites for preferential alkylation. Also encouraging this strategy is that the scope of nucleotide excision repair is very broad, and this system primarily recognizes DNA bubbles.Methods
A cationic xylyl (CAX) mass tag with limited nonpolarity was selected to increase the retention of polar adducts in reversed-phase high-performance liquid chromatography (HPLC) ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jul","modification":"2025-06-01T02:54:30.976Z","creation":"2024-11-15T15:58:49.873Z"},"accession":"S-EPMC10668917","cross_references":{"pubmed":["33821547"],"doi":["10.1002/rcm.9095"]}}