{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14"],"submitter":["Navarre C"],"pubmed_abstract":["Several recombinant proteins have been successfully produced in plants. This usually requires <i>Agrobacterium</i>-mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via <i>A. tumefaciens</i>, especially when the protein of interest is toxic to the bacteria. In particular, we found that a Tn3 transposon can be transferred from the pAL4404 Ti plasmid of <i>A. tumefaciens</i> LBA4404 into the expression cassette when using the widely adopted <i>35S</i> promoter, thereby damaging T-DNA and preventing correct expression of the gene of interest in <i>Nicotiana tabacum</i> BY-2 suspension cells."],"journal":["Frontiers in plant science"],"pagination":["1266775"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10679441"],"repository":["biostudies-literature"],"pubmed_title":["Issue when expressing a recombinant protein under the control of p<i>35S</i> in <i>Nicotiana tabacum</i> BY-2 cells."],"pmcid":["PMC10679441"],"pubmed_authors":["Orval R","Bailly N","Navarre C","Chaumont F","Peeters M"],"additional_accession":[]},"is_claimable":false,"name":"Issue when expressing a recombinant protein under the control of p<i>35S</i> in <i>Nicotiana tabacum</i> BY-2 cells.","description":"Several recombinant proteins have been successfully produced in plants. This usually requires <i>Agrobacterium</i>-mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via <i>A. tumefaciens</i>, especially when the protein of interest is toxic to the bacteria. In particular, we found that a Tn3 transposon can be transferred from the pAL4404 Ti plasmid of <i>A. tumefaciens</i> LBA4404 into the expression cassette when using the widely adopted <i>35S</i> promoter, thereby damaging T-DNA and preventing correct expression of the gene of interest in <i>Nicotiana tabacum</i> BY-2 suspension cells.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-05-29T00:18:57.944Z","creation":"2025-02-19T00:38:53.431Z"},"accession":"S-EPMC10679441","cross_references":{"pubmed":["38023881"],"doi":["10.3389/fpls.2023.1266775"]}}