biostudies-literatureLiu QNational Institute of Diabetes and Digestive and Kidney DiseasesNIDDK NIH HHSNHLBI NIH HHSNational Heart, Lung, and Blood InstituteLouisiana Board of RegentsNational Institutes of HealthNational Institute of General Medical SciencesNIGMS NIH HHS102703https://www.ebi.ac.uk/biostudies/studies/S-EPMC10679935biostudies-literatureUnknown4(4)Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).¹.STAR protocolsProgenitor cell isolation from mouse epididymal adipose tissue and sequencing library construction.PMC106799351R01HL157519P20GM130555R15 DK122383P20 GM130555BOR.Fu.LEQSF(2019-22)-RD-A-011R15DK122383R01 HL157519Stephens JMShiri MSimintiras CALi CAlkaifi FFu XLiu QFrancis JWang LDeng BLi YGao PZhang XZhao JSun JfalseProgenitor cell isolation from mouse epididymal adipose tissue and sequencing library construction.Here, we present a protocol to isolate progenitor cells from mouse epididymal visceral adipose tissue and construct bulk RNA and assay for transposase-accessible chromatin with sequencing (ATAC-seq) libraries. We describe steps for adipose tissue collection, cell isolation, and cell staining and sorting. We then detail procedures for both ATAC-seq and RNA sequencing library construction. This protocol can also be applied to other tissues and cell types directly or with minor modifications. For complete details on the use and execution of this protocol, please refer to Liu et al. (2023).¹.2023-01-01T00:00:00Z2023 Nov2025-04-18T16:47:22.822Z2025-04-07T04:12:57.567ZS-EPMC106799353794818610.1016/j.xpro.2023.102703