biostudies-literatureHuna AInstitut National Du Cancer318https://www.ebi.ac.uk/biostudies/studies/S-EPMC10680185biostudies-literatureUnknown42(1)<h4>Background</h4>TGFβ induces several cell phenotypes including senescence, a stable cell cycle arrest accompanied by a secretory program, and epithelial-mesenchymal transition (EMT) in normal epithelial cells. During carcinogenesis cells lose the ability to undergo senescence in response to TGFβ but they maintain an EMT, which can contribute to tumor progression. Our aim was to identify mechanisms promoting TGFβ-induced senescence escape.<h4>Methods</h4>In vitro experiments were performed with primary human mammary epithelial cells (HMEC) immortalized by hTert. For kinase library screen and modulation of gene expression retroviral transduction was used. To characterize gene expression, RNA microarray with GSEA analysis and RT-qPCR were used. For protein level and localization, Western bJournal of experimental & clinical cancer research : CRRSK3 switches cell fate: from stress-induced senescence to malignant progression.PMC10680185PLBio 18-144PLBio 15-013Huna ACoute YDefossez PABoissan MPakulska VMakulyte GSaintigny PRuisseaux CZhu KMartin NBernard DHernandez-Vargas HLodillinsky CFlaman JMfalseRSK3 switches cell fate: from stress-induced senescence to malignant progression.<h4>Background</h4>TGFβ induces several cell phenotypes including senescence, a stable cell cycle arrest accompanied by a secretory program, and epithelial-mesenchymal transition (EMT) in normal epithelial cells. During carcinogenesis cells lose the ability to undergo senescence in response to TGFβ but they maintain an EMT, which can contribute to tumor progression. Our aim was to identify mechanisms promoting TGFβ-induced senescence escape.<h4>Methods</h4>In vitro experiments were performed with primary human mammary epithelial cells (HMEC) immortalized by hTert. For kinase library screen and modulation of gene expression retroviral transduction was used. To characterize gene expression, RNA microarray with GSEA analysis and RT-qPCR were used. For protein level and localization, Western b2023-01-01T00:00:00Z2023 Nov2026-05-28T20:39:27.143Z2025-02-18T23:29:38.714ZS-EPMC106801853800875610.1186/s13046-023-02909-5