{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Kolakada D"],"funding":["NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Nonsense-mediated mRNA decay (NMD) is a network of pathways that degrades transcripts that undergo premature translation termination. In mammals, NMD can be divided into the exon junction complex (EJC)-enhanced and EJC-independent branches. Fluorescence- and luminescence-based reporters have long been effective tools to investigate NMD, yet existing reporters largely focus on the EJC-enhanced pathway. Here, we present a system of reporters for comparative studies of EJC-independent and EJC-enhanced NMD. This system also enables the study of NMD-associated outcomes such as premature termination codon (PTC) readthrough and truncated protein degradation. These reporters are compatible with fluorescence or luminescence-based readouts via transient transfection or stable integration. Using this"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.11.14.567061"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10680754"],"repository":["biostudies-literature"],"pubmed_title":["A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay."],"pmcid":["PMC10680754"],"funding_grant_id":["P30 CA046934","R35 GM133433","T32 GM136444"],"pubmed_authors":["Lore M","Jagannathan S","Li Z","Campbell AE","Kolakada D","Baquero Galvis L"],"additional_accession":[]},"is_claimable":false,"name":"A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay.","description":"Nonsense-mediated mRNA decay (NMD) is a network of pathways that degrades transcripts that undergo premature translation termination. In mammals, NMD can be divided into the exon junction complex (EJC)-enhanced and EJC-independent branches. Fluorescence- and luminescence-based reporters have long been effective tools to investigate NMD, yet existing reporters largely focus on the EJC-enhanced pathway. Here, we present a system of reporters for comparative studies of EJC-independent and EJC-enhanced NMD. This system also enables the study of NMD-associated outcomes such as premature termination codon (PTC) readthrough and truncated protein degradation. These reporters are compatible with fluorescence or luminescence-based readouts via transient transfection or stable integration. Using this","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-06-22T03:19:26.671Z","creation":"2024-10-18T18:16:05.358Z"},"accession":"S-EPMC10680754","cross_references":{"pubmed":["38014198"],"doi":["10.1101/2023.11.14.567061"]}}