biostudies-literatureUnknownKolakada DNCI NIH HHSNIGMS NIH HHSNonsense-mediated mRNA decay (NMD) is a network of pathways that degrades transcripts that undergo premature translation termination. In mammals, NMD can be divided into the exon junction complex (EJC)-enhanced and EJC-independent branches. Fluorescence- and luminescence-based reporters have long been effective tools to investigate NMD, yet existing reporters largely focus on the EJC-enhanced pathway. Here, we present a system of reporters for comparative studies of EJC-independent and EJC-enhanced NMD. This system also enables the study of NMD-associated outcomes such as premature termination codon (PTC) readthrough and truncated protein degradation. These reporters are compatible with fluorescence or luminescence-based readouts via transient transfection or stable integration. Using thisbioRxiv : the preprint server for biology2023.11.14.567061https://www.ebi.ac.uk/biostudies/studies/S-EPMC10680754biostudies-literatureA system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay.PMC10680754P30 CA046934R35 GM133433T32 GM136444Lore MJagannathan SLi ZCampbell AEKolakada DBaquero Galvis LfalseA system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay.Nonsense-mediated mRNA decay (NMD) is a network of pathways that degrades transcripts that undergo premature translation termination. In mammals, NMD can be divided into the exon junction complex (EJC)-enhanced and EJC-independent branches. Fluorescence- and luminescence-based reporters have long been effective tools to investigate NMD, yet existing reporters largely focus on the EJC-enhanced pathway. Here, we present a system of reporters for comparative studies of EJC-independent and EJC-enhanced NMD. This system also enables the study of NMD-associated outcomes such as premature termination codon (PTC) readthrough and truncated protein degradation. These reporters are compatible with fluorescence or luminescence-based readouts via transient transfection or stable integration. Using this2023-01-01T00:00:00Z2023 Nov2026-06-22T03:19:26.671Z2024-10-18T18:16:05.358ZS-EPMC106807543801419810.1101/2023.11.14.567061