biostudies-literatureRai SDepartment of Health Research, IndiaIndian Council of Medical Research1174951https://www.ebi.ac.uk/biostudies/studies/S-EPMC10684698biostudies-literatureUnknown17<h4>Background</h4>Parkinson's disease (PD) is an increasingly common neurodegenerative condition, which causes movement dysfunction and a broad range of non-motor symptoms. There is no molecular or biochemical diagnosis test for PD. The miRNAs are a class of small non-coding RNAs and are extensively studied owing to their altered expression in pathological states and facile harvesting and analysis techniques.<h4>Methods</h4>A total of 48 samples (16 each of PD, aged-matched, and young controls) were recruited. The small extracellular vesicles (sEVs) were isolated and validated using Western blot, transmission electron microscope, and nanoparticle tracking analysis. Small RNA isolation, library preparation, and small RNA sequencing followed by differential expression and targeted predictioFrontiers in neuroscienceCirculating plasma miR-23b-3p as a biomarker target for idiopathic Parkinson's disease: comparison with small extracellular vesicle miRNA.PMC106846982020-1194R.11012/21/2021-GIA/HRBharti PSRani KReddy TJInampudi KKPandey HCRani NKumar SRajan RSharma VModi GPSingh RYadav SRai SRastogi SVerma BKNikolajeff FGorai PKfalseCirculating plasma miR-23b-3p as a biomarker target for idiopathic Parkinson's disease: comparison with small extracellular vesicle miRNA.<h4>Background</h4>Parkinson's disease (PD) is an increasingly common neurodegenerative condition, which causes movement dysfunction and a broad range of non-motor symptoms. There is no molecular or biochemical diagnosis test for PD. The miRNAs are a class of small non-coding RNAs and are extensively studied owing to their altered expression in pathological states and facile harvesting and analysis techniques.<h4>Methods</h4>A total of 48 samples (16 each of PD, aged-matched, and young controls) were recruited. The small extracellular vesicles (sEVs) were isolated and validated using Western blot, transmission electron microscope, and nanoparticle tracking analysis. Small RNA isolation, library preparation, and small RNA sequencing followed by differential expression and targeted predictio2023-01-01T00:00:00Z20232026-06-03T12:24:34.103Z2025-04-06T09:39:29.801ZS-EPMC106846983803354710.3389/fnins.2023.1174951