biostudies-literatureSu YNational Natural Science Foundation of ShanghaiNational Natural Science Foundation of China326https://www.ebi.ac.uk/biostudies/studies/S-EPMC10685607biostudies-literatureUnknown42(1)<h4>Background</h4>As a small G protein of Ras family, Ras-like-without-CAAX-1 (RIT1) plays a critical role in various tumors. Our previous study has demonstrated the involvement of RIT1 in promoting malignant progression of hepatocellular carcinoma (HCC). However, its underlying mechanism remains unclear.<h4>Methods</h4>Gene set enrichment analysis (GSEA) was conducted in the TCGA LIHC cohort to investigate the underlying biological mechanism of RIT1. Live cell imaging, immunofluorescence (IF) and flow cytometry assays were used to verify biological function of RIT1 in HCC mitosis. Subcutaneous xenografting of human HCC cells in BALB/c nude mice was utilized to assess tumor proliferation in vivo. RNA-seq, co-immunoprecipitation (Co-IP), mass spectrometry analyses, western blot and IF assaJournal of experimental & clinical cancer research : CRRIT1 regulates mitosis and promotes proliferation by interacting with SMC3 and PDS5 in hepatocellular carcinoma.PMC1068560720ZR145400082172904Ge CLi HJin WGeng QLi JJiang SYu JLiu TZhang SMao LZhou XCui YLin HZhao FSu YHou HWu YChen TfalseRIT1 regulates mitosis and promotes proliferation by interacting with SMC3 and PDS5 in hepatocellular carcinoma.<h4>Background</h4>As a small G protein of Ras family, Ras-like-without-CAAX-1 (RIT1) plays a critical role in various tumors. Our previous study has demonstrated the involvement of RIT1 in promoting malignant progression of hepatocellular carcinoma (HCC). However, its underlying mechanism remains unclear.<h4>Methods</h4>Gene set enrichment analysis (GSEA) was conducted in the TCGA LIHC cohort to investigate the underlying biological mechanism of RIT1. Live cell imaging, immunofluorescence (IF) and flow cytometry assays were used to verify biological function of RIT1 in HCC mitosis. Subcutaneous xenografting of human HCC cells in BALB/c nude mice was utilized to assess tumor proliferation in vivo. RNA-seq, co-immunoprecipitation (Co-IP), mass spectrometry analyses, western blot and IF assa2023-01-01T00:00:00Z2023 Nov2025-04-04T19:00:11.369Z2025-02-19T04:43:58.735ZS-EPMC106856073801747910.1186/s13046-023-02892-x