biostudies-literatureUnknown8(4)Ping Jl-Tyrosine, an aromatic non-essential amino acid, is the raw material for many important chemical products, including levodopa, resveratrol, and hydroxytyrosol. It is widely used in the food, drug, and chemical industries. There are many studies on the synthesis of l-tyrosine by microorganisms, however, the low titer of l-tyrosine limited the industrial large-scale production. In order to enhance l-tyrosine production in <i>Escherichia coli</i>, the expression of key enzymes in the shikimate pathway was up- or down-regulated. The l-tyrosine transport system and the acetic acid biosynthesis pathway were modified to further enhance l-tyrosine production. In addition, the phosphoketolase pathway was introduced in combination with cofactor engineering to redirect carbon flux to the shikimate pSynthetic and systems biotechnology724-731https://www.ebi.ac.uk/biostudies/studies/S-EPMC10686809biostudies-literatureSynergetic engineering of <i>Escherichia coli</i> for efficient production of l-tyrosine.PMC10686809Ping JQin ZWang LZhou ZZhou JfalseSynergetic engineering of <i>Escherichia coli</i> for efficient production of l-tyrosine.l-Tyrosine, an aromatic non-essential amino acid, is the raw material for many important chemical products, including levodopa, resveratrol, and hydroxytyrosol. It is widely used in the food, drug, and chemical industries. There are many studies on the synthesis of l-tyrosine by microorganisms, however, the low titer of l-tyrosine limited the industrial large-scale production. In order to enhance l-tyrosine production in <i>Escherichia coli</i>, the expression of key enzymes in the shikimate pathway was up- or down-regulated. The l-tyrosine transport system and the acetic acid biosynthesis pathway were modified to further enhance l-tyrosine production. In addition, the phosphoketolase pathway was introduced in combination with cofactor engineering to redirect carbon flux to the shikimate p2023-01-01T00:00:00Z2023 Dec2025-04-04T19:00:34.581Z2025-04-04T19:00:34.581ZS-EPMC106868093803375610.1016/j.synbio.2023.10.005