biostudies-literatureUnknownPlitt MHNIMH NIH HHSNINDS NIH HHSRapid delivery of glutamate receptors to the postsynaptic membrane via vesicle fusion is a central component of synaptic plasticity. However, it is unknown how this process supports specific neural computations during behavior. To bridge this gap, we combined conditional genetic deletion of a component of the postsynaptic membrane fusion machinery, Syntaxin3 (Stx3), in hippocampal CA1 neurons of mice with population <i>in vivo</i> calcium imaging. This approach revealed that Stx3 is necessary for forming the neural dynamics that support novelty processing, spatial reward memory and offline memory consolidation. In contrast, CA1 Stx3 was dispensable for maintaining aspects of the neural code that exist presynaptic to CA1 such as representations of context and space. Thus, manipulating postsbioRxiv : the preprint server for biology2023.11.20.567978https://www.ebi.ac.uk/biostudies/studies/S-EPMC10690209biostudies-literatureHippocampal place code plasticity in CA1 requires postsynaptic membrane fusion.PMC10690209R01 MH126904R01 MH130452U19 NS118284Kaganovsky KSudhof TCPlitt MHGiocomo LMfalseHippocampal place code plasticity in CA1 requires postsynaptic membrane fusion.Rapid delivery of glutamate receptors to the postsynaptic membrane via vesicle fusion is a central component of synaptic plasticity. However, it is unknown how this process supports specific neural computations during behavior. To bridge this gap, we combined conditional genetic deletion of a component of the postsynaptic membrane fusion machinery, Syntaxin3 (Stx3), in hippocampal CA1 neurons of mice with population <i>in vivo</i> calcium imaging. This approach revealed that Stx3 is necessary for forming the neural dynamics that support novelty processing, spatial reward memory and offline memory consolidation. In contrast, CA1 Stx3 was dispensable for maintaining aspects of the neural code that exist presynaptic to CA1 such as representations of context and space. Thus, manipulating posts2023-01-01T00:00:00Z2023 Nov2026-06-26T03:16:26.576Z2025-04-25T17:21:24.947ZS-EPMC106902093804536210.1101/2023.11.20.567978