biostudies-literatureLiang JNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaBeijing Science and Technology Planning Project157https://www.ebi.ac.uk/biostudies/studies/S-EPMC10691013biostudies-literatureUnknown18(1)<h4>Background</h4>Acute-on-chronic liver failure (ACLF) is a refractory disease with high mortality, which is characterized by a pathophysiological process of inflammation-related dysfunction of energy metabolism. Jieduan-Niwan formula (JDNWF) is a eutherapeutic Chinese medicine formula for ACLF. However, the intrinsic mechanism of its anti-ACLF effect still need to be studied systematically.<h4>Purpose</h4>This study aimed to investigate the mechanism of JDNWF against ACLF based on altered substance metabolic profile in ACLF the expression levels of related molecules.<h4>Materials and methods</h4>The chemical characteristics of JDNWF were characterized using ultra performance liquid chromatography (UPLC) coupled with triple quadrupole mass spectrometry. Wistar rats subjected to a long-teChinese medicineLiver metabolomics reveals potential mechanism of Jieduan-Niwan formula against acute-on-chronic liver failure (ACLF) by improving mitochondrial damage and TCA cycle.PMC10691013820742372018YFC1704102JJ-2020-51Ma RLiang JGao YWei XDu YWang HZhang QHou WfalseLiver metabolomics reveals potential mechanism of Jieduan-Niwan formula against acute-on-chronic liver failure (ACLF) by improving mitochondrial damage and TCA cycle.<h4>Background</h4>Acute-on-chronic liver failure (ACLF) is a refractory disease with high mortality, which is characterized by a pathophysiological process of inflammation-related dysfunction of energy metabolism. Jieduan-Niwan formula (JDNWF) is a eutherapeutic Chinese medicine formula for ACLF. However, the intrinsic mechanism of its anti-ACLF effect still need to be studied systematically.<h4>Purpose</h4>This study aimed to investigate the mechanism of JDNWF against ACLF based on altered substance metabolic profile in ACLF the expression levels of related molecules.<h4>Materials and methods</h4>The chemical characteristics of JDNWF were characterized using ultra performance liquid chromatography (UPLC) coupled with triple quadrupole mass spectrometry. Wistar rats subjected to a long-te2023-01-01T00:00:00Z2023 Nov2025-04-05T11:45:12.471Z2025-04-05T11:45:12.471ZS-EPMC106910133803715010.1186/s13020-023-00858-x