{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramsak M"],"funding":["Javna Agencija za Raziskovalno Dejavnost RS","NIGMS NIH HHS"],"pagination":["7973"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10693550"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Membraneless liquid compartments based on phase-separating biopolymers have been observed in diverse cell types and attributed to weak multivalent interactions predominantly based on intrinsically disordered domains. The design of liquid-liquid phase separated (LLPS) condensates based on de novo designed tunable modules that interact in a well-understood, controllable manner could improve our understanding of this phenomenon and enable the introduction of new features. Here we report the construction of CC-LLPS in mammalian cells, based on designed coiled-coil (CC) dimer-forming modules, where the stability of CC pairs, their number, linkers, and sequential arrangement govern the transition between diffuse, liquid and immobile condensates and are corroborated by coarse-grained molecular simulations. Through modular design, we achieve multiple coexisting condensates, chemical regulation of LLPS, condensate fusion, formation from either one or two polypeptide components or LLPS regulation by a third polypeptide chain. These findings provide further insights into the principles underlying LLPS formation and a design platform for controlling biological processes."],"journal":["Nature communications"],"pubmed_title":["Programmable de novo designed coiled coil-mediated phase separation in mammalian cells."],"pmcid":["PMC10693550"],"funding_grant_id":["T32 GM065103","P4-0176, P3-0289","T32 GM145437"],"pubmed_authors":["Hough LE","Shirts MR","Ramsak M","Ramirez DA","Elersic Filipic K","Vidmar S","Anderluh G","Jerala R"],"additional_accession":[]},"is_claimable":false,"name":"Programmable de novo designed coiled coil-mediated phase separation in mammalian cells.","description":"Membraneless liquid compartments based on phase-separating biopolymers have been observed in diverse cell types and attributed to weak multivalent interactions predominantly based on intrinsically disordered domains. The design of liquid-liquid phase separated (LLPS) condensates based on de novo designed tunable modules that interact in a well-understood, controllable manner could improve our understanding of this phenomenon and enable the introduction of new features. Here we report the construction of CC-LLPS in mammalian cells, based on designed coiled-coil (CC) dimer-forming modules, where the stability of CC pairs, their number, linkers, and sequential arrangement govern the transition between diffuse, liquid and immobile condensates and are corroborated by coarse-grained molecular simulations. Through modular design, we achieve multiple coexisting condensates, chemical regulation of LLPS, condensate fusion, formation from either one or two polypeptide components or LLPS regulation by a third polypeptide chain. These findings provide further insights into the principles underlying LLPS formation and a design platform for controlling biological processes.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2026-06-22T03:16:59.668Z","creation":"2025-02-19T01:17:08.378Z"},"accession":"S-EPMC10693550","cross_references":{"pubmed":["38042897"],"doi":["10.1038/s41467-023-43742-w"]}}