{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kochan TJ"],"funding":["American Heart Association","NIAID NIH HHS"],"pagination":["7962"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10693551"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Klebsiella pneumoniae has been classified into two types, classical K. pneumoniae (cKP) and hypervirulent K. pneumoniae (hvKP). cKP isolates are highly diverse and important causes of nosocomial infections; they include globally disseminated antibiotic-resistant clones. hvKP isolates are sensitive to most antibiotics but are highly virulent, causing community-acquired infections in healthy individuals. The virulence phenotype of hvKP is associated with pathogenicity loci responsible for siderophore and hypermucoid capsule production. Recently, convergent strains of K. pneumoniae, which possess features of both cKP and hvKP, have emerged and are cause of much concern. Here, we screen the genomes of 2,608 multidrug-resistant K. pneumoniae isolates from the United States and identify 47 convergent isolates. We perform phenotypic and genomic characterization of 12 representative isolates. These 12 convergent isolates contain a variety of antimicrobial resistance plasmids and virulence plasmids. Most convergent isolates contain aerobactin biosynthesis genes and produce more siderophores than cKP isolates but not more capsule. Unexpectedly, only 1 of the 12 tested convergent isolates has a level of virulence consistent with hvKP isolates in a murine pneumonia model. These findings suggest that additional studies should be performed to clarify whether convergent strains are indeed more virulent than cKP in mouse and human infections."],"journal":["Nature communications"],"pubmed_title":["Klebsiella pneumoniae clinical isolates with features of both multidrug-resistance and hypervirulence have unexpectedly low virulence."],"pmcid":["PMC10693551"],"funding_grant_id":["K24 AI104831","R01 AI173064","837089","R01 AI118257","T32 AI095207","T32 AI007476","R21 AI164254","R21 AI153953"],"pubmed_authors":["VanGosen EM","Ward TJ","Musser JM","Mitra SD","Cheung BH","Hauser AR","Nelson JA","Chen L","Long SW","Wunderink RG","Axline CMR","van Duin D","Kreiswirth BN","Bulman ZP","Valdes A","Vessely MB","Mills JO","Nozick SH","Kochan TJ","Ozer EA","Lebrun-Corbin M","Medernach RL"],"additional_accession":[]},"is_claimable":false,"name":"Klebsiella pneumoniae clinical isolates with features of both multidrug-resistance and hypervirulence have unexpectedly low virulence.","description":"Klebsiella pneumoniae has been classified into two types, classical K. pneumoniae (cKP) and hypervirulent K. pneumoniae (hvKP). cKP isolates are highly diverse and important causes of nosocomial infections; they include globally disseminated antibiotic-resistant clones. hvKP isolates are sensitive to most antibiotics but are highly virulent, causing community-acquired infections in healthy individuals. The virulence phenotype of hvKP is associated with pathogenicity loci responsible for siderophore and hypermucoid capsule production. Recently, convergent strains of K. pneumoniae, which possess features of both cKP and hvKP, have emerged and are cause of much concern. Here, we screen the genomes of 2,608 multidrug-resistant K. pneumoniae isolates from the United States and identify 47 convergent isolates. We perform phenotypic and genomic characterization of 12 representative isolates. These 12 convergent isolates contain a variety of antimicrobial resistance plasmids and virulence plasmids. Most convergent isolates contain aerobactin biosynthesis genes and produce more siderophores than cKP isolates but not more capsule. Unexpectedly, only 1 of the 12 tested convergent isolates has a level of virulence consistent with hvKP isolates in a murine pneumonia model. These findings suggest that additional studies should be performed to clarify whether convergent strains are indeed more virulent than cKP in mouse and human infections.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2026-06-22T03:17:17.671Z","creation":"2025-02-19T01:16:32.553Z"},"accession":"S-EPMC10693551","cross_references":{"pubmed":["38042959"],"doi":["10.1038/s41467-023-43802-1"]}}