biostudies-literatureUnknown98Mo Y<h4>Background</h4>HIV-1-associated immune activation drives CD4⁺ T cell depletion and the development of acquired immunodeficiency syndrome. We aimed to determine the role of nicotinamide mononucleotide (NMN), the direct precursor of nicotinamide adenine dinucleotide (NAD) co-enzyme, in CD4⁺ T cell modulation during HIV-1 infection.<h4>Methods</h4>We examined HIV-1 integrated DNA or transcribed RNA, intracellular p24 protein, and T cell activation markers in CD4⁺ T cells including in vitro HIV-1-infected cells, reactivated patient-derived cells, and in HIV-1-infected humanized mice, under NMN treatment. RNA-seq and CyTOF analyses were used for investigating the effect of NMN on CD4⁺ T cells.<h4>Findings</h4>We found that NMN increased the intracEBioMedicine104877https://www.ebi.ac.uk/biostudies/studies/S-EPMC10694053biostudies-literatureNicotinamide mononucleotide impacts HIV-1 infection by modulating immune activation in T lymphocytes and humanized mice.PMC10694053Liu LZhou RHuang HYim LYYue MWang HZhou YLui GCWang JLuk TYLim CYHYu CChen ZPeng QSun HMo YSong YfalseNicotinamide mononucleotide impacts HIV-1 infection by modulating immune activation in T lymphocytes and humanized mice.<h4>Background</h4>HIV-1-associated immune activation drives CD4⁺ T cell depletion and the development of acquired immunodeficiency syndrome. We aimed to determine the role of nicotinamide mononucleotide (NMN), the direct precursor of nicotinamide adenine dinucleotide (NAD) co-enzyme, in CD4⁺ T cell modulation during HIV-1 infection.<h4>Methods</h4>We examined HIV-1 integrated DNA or transcribed RNA, intracellular p24 protein, and T cell activation markers in CD4⁺ T cells including in vitro HIV-1-infected cells, reactivated patient-derived cells, and in HIV-1-infected humanized mice, under NMN treatment. RNA-seq and CyTOF analyses were used for investigating the effect of NMN on CD4⁺ T cells.<h4>Findings</h4>We found that NMN increased the intrac2023-01-01T00:00:00Z2023 Dec2026-05-29T07:38:17.055Z2025-02-19T01:17:51.172ZS-EPMC106940533798079410.1016/j.ebiom.2023.104877