biostudies-literatureUnknown13Strikic ARenal cell carcinoma (RCC) represents around 3% of all cancers, with the most frequent histological types being clear-cell RCC (ccRCC), followed by papillary (pRCC) and chromophobe (chRCC). Hypoxia-inducible factors (HIFs), which promote the expression of various target genes, including vascular endothelial growth factor (VEGF) and the high- affinity glucose transporter 1, have an important role in the pathogenesis of RCC. This study investigated the immunohistochemical expression of HIF-1α and VEGF-A, showing significantly higher HIF-1α nuclear expression in pRCC compared to ccRCC, while there was no significant difference in VEGF-A protein expression between the analyzed histological RCC subtypes. The quantitative reverse transcription polymerase chain reaction for <i>HIF1A</i> showed no statistical difference between histological types. Data from publicly available RNA sequencing databases were analyzed and showed that, compared to healthy kidney tissue, <i>VEGFA</i> was significantly up-regulated in ccRCC and significantly down-regulated in pRCC. The comparison between histological subtypes of RCC revealed that <i>VEGFA</i> was significantly up-regulated in ccRCC compared to both pRCC and chRCC. There was no statistically significant difference in survival time between <i>HIF1A</i> high- and low-expression groups of patients. As for <i>VEGFA</i> expression, pRCC patients with low expression had a significantly higher survival rate compared to patients with high <i>VEGFA</i> expression.Frontiers in oncology1287239https://www.ebi.ac.uk/biostudies/studies/S-EPMC10694430biostudies-literatureDifferential expression of <i>HIF1A</i> and its downstream target <i>VEGFA</i> in the main subtypes of renal cell carcinoma and their impact on patient survival.PMC10694430Kelam NTomas SZDolonga PKokeza JVukoja MOgorevc MStrikic AfalseDifferential expression of <i>HIF1A</i> and its downstream target <i>VEGFA</i> in the main subtypes of renal cell carcinoma and their impact on patient survival.Renal cell carcinoma (RCC) represents around 3% of all cancers, with the most frequent histological types being clear-cell RCC (ccRCC), followed by papillary (pRCC) and chromophobe (chRCC). Hypoxia-inducible factors (HIFs), which promote the expression of various target genes, including vascular endothelial growth factor (VEGF) and the high- affinity glucose transporter 1, have an important role in the pathogenesis of RCC. This study investigated the immunohistochemical expression of HIF-1α and VEGF-A, showing significantly higher HIF-1α nuclear expression in pRCC compared to ccRCC, while there was no significant difference in VEGF-A protein expression between the analyzed histological RCC subtypes. The quantitative reverse transcription polymerase chain reaction for <i>HIF1A</i> showed no statistical difference between histological types. Data from publicly available RNA sequencing databases were analyzed and showed that, compared to healthy kidney tissue, <i>VEGFA</i> was significantly up-regulated in ccRCC and significantly down-regulated in pRCC. The comparison between histological subtypes of RCC revealed that <i>VEGFA</i> was significantly up-regulated in ccRCC compared to both pRCC and chRCC. There was no statistically significant difference in survival time between <i>HIF1A</i> high- and low-expression groups of patients. As for <i>VEGFA</i> expression, pRCC patients with low expression had a significantly higher survival rate compared to patients with high <i>VEGFA</i> expression.2023-01-01T00:00:00Z20232025-05-18T11:51:33.96Z2025-05-18T11:51:33.96ZS-EPMC106944303805365510.3389/fonc.2023.1287239