{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Broxmeyer HE"],"funding":["Indiana University","NIDDK NIH HHS","NHLBI NIH HHS","National Cancer Institute","U.S. Public Health Service","NCI NIH HHS","National Institutes of Health","National Heart Lung and Blood Institute","NIDDK","NIH HHS"],"pagination":["101259"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10694620"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(11)"],"pubmed_abstract":["Umbilical cord blood transplantation is a life-saving treatment for malignant and non-malignant hematologic disorders. It remains unclear how long cryopreserved units remain functional, and the length of cryopreservation is often used as a criterion to exclude older units. We demonstrate that long-term cryopreserved cord blood retains similar numbers of hematopoietic stem and progenitor cells compared with fresh and recently cryopreserved cord blood units. Long-term cryopreserved units contain highly functional cells, yielding robust engraftment in mouse transplantation models. We also leverage differences between units to examine gene programs associated with better engraftment. Transcriptomic analyses reveal that gene programs associated with lineage determination and oxidative stress are enriched in high engrafting cord blood, revealing potential molecular markers to be used as potency markers for cord blood unit selection regardless of length of cryopreservation. In summary, cord blood units cryopreserved for extended periods retain engrafting potential and can potentially be used for patient treatment."],"journal":["Cell reports. Medicine"],"pubmed_title":["Insights into highly engraftable hematopoietic cells from 27-year cryopreserved umbilical cord blood."],"pmcid":["PMC10694620"],"funding_grant_id":["R35 HL139599","S10 OD012270","R01 HL155574","P30 CA082709","U54 DK106846","K99 HL166790","1S10D012270","F32 HL160072"],"pubmed_authors":["Weinberg RS","Kaplan MH","Van't Hof W","Ropa J","Broxmeyer HE","Hillyer CD","Cooper S","Luchsinger LL","Capitano ML","Jimenez A","Frenet EM"],"additional_accession":[]},"is_claimable":false,"name":"Insights into highly engraftable hematopoietic cells from 27-year cryopreserved umbilical cord blood.","description":"Umbilical cord blood transplantation is a life-saving treatment for malignant and non-malignant hematologic disorders. It remains unclear how long cryopreserved units remain functional, and the length of cryopreservation is often used as a criterion to exclude older units. We demonstrate that long-term cryopreserved cord blood retains similar numbers of hematopoietic stem and progenitor cells compared with fresh and recently cryopreserved cord blood units. Long-term cryopreserved units contain highly functional cells, yielding robust engraftment in mouse transplantation models. We also leverage differences between units to examine gene programs associated with better engraftment. Transcriptomic analyses reveal that gene programs associated with lineage determination and oxidative stress are enriched in high engrafting cord blood, revealing potential molecular markers to be used as potency markers for cord blood unit selection regardless of length of cryopreservation. In summary, cord blood units cryopreserved for extended periods retain engrafting potential and can potentially be used for patient treatment.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-05-29T00:41:18.718Z","creation":"2025-04-06T05:43:20.04Z"},"accession":"S-EPMC10694620","cross_references":{"pubmed":["37913777"],"doi":["10.1016/j.xcrm.2023.101259"]}}