biostudies-literatureOlivier FABMonash UniversityNational Health and Medical Research CouncilAustralian GovernmentAustralian Research Council102737https://www.ebi.ac.uk/biostudies/studies/S-EPMC10694764biostudies-literatureUnknown4(4)Population-level dynamics of host-pathogen interactions can be characterized using quantitative live-cell imaging. Here, we present a protocol for infecting macrophages with the fungal pathogen Candida albicans in vitro and quantitative live-cell imaging of immune and pathogen responses. We describe steps for detailed image analysis and provide resources for quantification of phagocytosis and pathogen escape, as well as macrophage membrane permeabilization and viability. This protocol is modifiable for applications with a range of pathogens, immune cell types, and host-pathogen mechanisms. For complete details on the use and execution of this protocol, please refer to Olivier et al.¹.STAR protocolsQuantitative live-cell imaging of Candida albicans escape from immune phagocytes.PMC10694764FT190100733APP1158678Traven AOlivier FABfalseQuantitative live-cell imaging of Candida albicans escape from immune phagocytes.Population-level dynamics of host-pathogen interactions can be characterized using quantitative live-cell imaging. Here, we present a protocol for infecting macrophages with the fungal pathogen Candida albicans in vitro and quantitative live-cell imaging of immune and pathogen responses. We describe steps for detailed image analysis and provide resources for quantification of phagocytosis and pathogen escape, as well as macrophage membrane permeabilization and viability. This protocol is modifiable for applications with a range of pathogens, immune cell types, and host-pathogen mechanisms. For complete details on the use and execution of this protocol, please refer to Olivier et al.¹.2023-01-01T00:00:00Z2023 Dec2026-06-17T06:47:39.57Z2026-06-17T03:09:59.256ZS-EPMC106947643798056710.1016/j.xpro.2023.102737