biostudies-literatureDenolly SEuropean Research CouncilDeutsche Forschungsgemeinschaft (German Research Foundation)8045https://www.ebi.ac.uk/biostudies/studies/S-EPMC10698153biostudies-literatureUnknown14(1)Zika virus (ZIKV) has emerged as a global health issue, yet neither antiviral therapy nor a vaccine are available. ZIKV is an enveloped RNA virus, replicating in the cytoplasm in close association with ER membranes. Here, we isolate ER membranes from ZIKV-infected cells and determine their proteome. Forty-six host cell factors are enriched in ZIKV remodeled membranes, several of these having a role in redox and methylation pathways. Four proteins are characterized in detail: thioredoxin reductase 1 (TXNRD1) contributing to folding of disulfide bond containing proteins and modulating ZIKV secretion; aldo-keto reductase family 1 member C3 (AKR1C3), regulating capsid protein abundance and thus, ZIKV assembly; biliverdin reductase B (BLVRB) involved in ZIKV induced lipid peroxidation and increNature communicationsZika virus remodelled ER membranes contain proviral factors involved in redox and methylation pathways.PMC10698153TRR179 (TP11))817798240245660-SFB1129SPP2306499982526-BARosinski ANKritsiligkou PJoecks SDick TPStukalov ADenolly SBarayeu UPichlmair ABartenschlager RfalseZika virus remodelled ER membranes contain proviral factors involved in redox and methylation pathways.Zika virus (ZIKV) has emerged as a global health issue, yet neither antiviral therapy nor a vaccine are available. ZIKV is an enveloped RNA virus, replicating in the cytoplasm in close association with ER membranes. Here, we isolate ER membranes from ZIKV-infected cells and determine their proteome. Forty-six host cell factors are enriched in ZIKV remodeled membranes, several of these having a role in redox and methylation pathways. Four proteins are characterized in detail: thioredoxin reductase 1 (TXNRD1) contributing to folding of disulfide bond containing proteins and modulating ZIKV secretion; aldo-keto reductase family 1 member C3 (AKR1C3), regulating capsid protein abundance and thus, ZIKV assembly; biliverdin reductase B (BLVRB) involved in ZIKV induced lipid peroxidation and incre2023-01-01T00:00:00Z2023 Dec2026-05-28T20:04:24.07Z2024-11-06T04:59:56.512ZS-EPMC106981533805281710.1038/s41467-023-43665-6